Morsiani E, Lacy P E
Department of Pathology, Washington University School of Medicine, St. Louis, Mo.
Eur Surg Res. 1990;22(2):78-85. doi: 10.1159/000129086.
Catfish (Ictalurus punctatus) islets were transplanted into the renal subcapsular space of diabetic C57BL/6J mice. The xenograft mean survival time (MST) of islets cultured for 6 days at 24 degrees C was 4.9 +/- 0.5 days, while xenotransplantation of 1-hour-cultured islets did not restore normoglycemia in the diabetic mice. A modest but significant prolongation of the MST was obtained by administration of anti-L3T4 monoclonal antibody to the recipients (13.4 +/- 2.1 days). Anti-L3T4 in conjunction with low-temperature culture did not produce a further increase of the MST (9.1 +/- 2.7 days). These findings indicate that catfish islets may be a source of islet tissue for transplantation in diabetic recipients. However, procedures that demonstrated to be effective in prolonging the MST of xenografts transplanted across wide species barrier, such as low-temperature culture and anti-L3T4 treatment, failed to prolong markedly the xenograft survival. Further experimental work is needed to study the possibility of improving islets survival after xenotransplantation across distant species barrier (fish to mouse).
将鲶鱼（斑点叉尾鮰）胰岛移植到糖尿病C57BL/6J小鼠的肾被膜下间隙。在24摄氏度下培养6天的胰岛异种移植物平均存活时间（MST）为4.9±0.5天，而培养1小时的胰岛异种移植未能使糖尿病小鼠恢复正常血糖。给受体注射抗L3T4单克隆抗体可适度但显著延长MST（13.4±2.1天）。抗L3T4与低温培养联合使用并未使MST进一步增加（9.1±2.7天）。这些发现表明鲶鱼胰岛可能是糖尿病受体移植胰岛组织的一个来源。然而，已证明在延长跨广泛物种屏障移植的异种移植物MST方面有效的方法，如低温培养和抗L3T4治疗，未能显著延长异种移植物的存活时间。需要进一步的实验工作来研究提高跨远距离物种屏障（鱼到小鼠）异种移植后胰岛存活的可能性。
