Lacy P E, Ricordi C, Finke E H
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Transplantation. 1989 May;47(5):761-6. doi: 10.1097/00007890-198905000-00002.
Rat, hamster, and rabbit islets were transplanted into diabetic C57BL/B6 mice. The effect on islet xenograft survival of low-temperature culture of the donor islets, alpha L3T4 treatment of the recipients for seven days, and transplantation of the grafts either in the renal capsule or in the liver via the portal vein was determined. Renal capsule transplants of control rat, hamster, and rabbit islets cultured at 37 degrees C for one day produced normoglycemia in the recipients, with the mean survival time (MST) of the grafts ranging from 14 to 19 days. Low temperature culture alone did not produce a significant increase in the survival time. Treatment of the recipients with alpha L3T4 produced a marked prolongation of xenograft survival for all three species receiving renal subcapsular transplants of control or low temperature cultured islets. The range of MST* was from 34 to 46 days. The intrahepatic site produced an even further prolongation of the survival of concordant rat islet xenografts treated in this manner, with 60% of the recipients still normoglycemic at 100 days after transplantation. This enhancing effect of the intrahepatic site on survival did not occur with the discordant xenografts of hamster and rabbit islets.
将大鼠、仓鼠和兔的胰岛移植到糖尿病C57BL/B6小鼠体内。确定了供体胰岛低温培养、受体接受αL3T4治疗7天以及将移植物通过门静脉移植到肾被膜或肝脏中对胰岛异种移植物存活的影响。在37℃培养一天的对照大鼠、仓鼠和兔胰岛的肾被膜移植使受体血糖正常，移植物的平均存活时间（MST）为14至19天。单独低温培养并未显著延长存活时间。用αL3T4治疗受体可显著延长接受对照或低温培养胰岛肾被膜下移植的所有三个物种的异种移植物存活时间。MST范围为34至46天。肝内移植部位可进一步延长以这种方式处理的一致性大鼠胰岛异种移植物的存活时间，60%的受体在移植后100天仍血糖正常。肝内移植部位对存活的这种增强作用在仓鼠和兔胰岛的非一致性异种移植物中未出现。
