Department of Clinical and Experimental Medicine and Surgery, F Magrassi-A Lanzara, Second University of Naples, Via Pansini 5, 80131 Naples, Italy.
J Endocrinol Invest. 2011 Dec;34(11):831-4. doi: 10.3275/7414. Epub 2010 Dec 15.
Chemokines play a key role in the recruitment of the immune cells into the autoimmune process. Thus, the simultaneous evaluation of circulating levels of Th1-related chemokines, such as CX chemokine ligand 10 (CXCL10) and macrophage inflammatory proteins 1α (CCL3/MIP-1α), and Th2-related chemokines, such as macrophage inflammatory proteins 1 β (CCL4/MIP-1β) could be useful in the approach to some autoimmune diseases, including autoimmune Addison's disease (AAD).
To evaluate plasmatic levels of MIP-1α, MIP-1β, CXCL10 and adrenocortical antibodies in patients with AAD under treatment with corticosteroids.
Twelve women and 5 men (group 1) were divided in 2 subgroups: 9 subjects with isolated AAD (group 1a) and 8 with AAD associated with chronic autoimmune thyroiditis (group 1b). MIP-1α, MIP- 1β and CXCL10 were evaluated in the serum of all patients and in 20 healthy controls, using a system for microarray suspension.
The levels of MIP-1α, MIP-1β and CXCL10 resulted significantly increased vs controls (p<0.001). An inverse significant correlation between the serum levels of MIP- 1β and the duration of the disease was observed.
High levels of MIP-1α and MIP-1β associated with increased levels of CXCL10 in AAD seem to indicate a role of these chemokines in the autoimmune pathology of adrenal gland through the recruitment in loco of Th1 and Th2 cells. The simultaneous measurement of Th1-related chemokines (CXCL10 and MIP-1α) and of Th2-related chemokine MIP-1β in the serum of patients with AAD would sustain a novel preliminary hypothesis on the immune microenvironment of chronic autoimmune inflammation within adrenal glands.
趋化因子在免疫细胞招募到自身免疫过程中起着关键作用。因此，同时评估循环中 Th1 相关趋化因子，如 CX 趋化因子配体 10（CXCL10）和巨噬细胞炎性蛋白 1α（CCL3/MIP-1α），以及 Th2 相关趋化因子，如巨噬细胞炎性蛋白 1β（CCL4/MIP-1β）的水平，可能对一些自身免疫性疾病的治疗有用，包括自身免疫性肾上腺皮质功能减退症（AAD）。
评估皮质类固醇治疗的 AAD 患者的血浆 MIP-1α、MIP-1β、CXCL10 和肾上腺皮质抗体水平。
12 名女性和 5 名男性（组 1）分为 2 个亚组：9 名单纯 AAD 患者（组 1a）和 8 名 AAD 合并慢性自身免疫性甲状腺炎患者（组 1b）。使用微阵列悬浮液系统评估所有患者和 20 名健康对照者的血清中 MIP-1α、MIP-1β 和 CXCL10。
MIP-1α、MIP-1β 和 CXCL10 的水平与对照组相比显著升高（p<0.001）。观察到血清 MIP-1β 水平与疾病持续时间呈负相关。
AAD 中 MIP-1α 和 MIP-1β 水平升高，以及 CXCL10 水平升高，表明这些趋化因子在通过募集 Th1 和 Th2 细胞在肾上腺的自身免疫病理中起作用。在 AAD 患者的血清中同时测量 Th1 相关趋化因子（CXCL10 和 MIP-1α）和 Th2 相关趋化因子 MIP-1β，将支持关于肾上腺内慢性自身免疫炎症免疫微环境的新的初步假设。
