Burgos M, Olmos D M, Jiménez R, Sánchez A, Díaz de la Guardia R
Departamento de Biología Animal, Ecología y Genética, Facultad de Ciencias, Universidad de Granada, Spain.
Genetica. 1990;81(1):11-6. doi: 10.1007/BF00055231.
Three different fluorochrome and specific counterstain combination (DAPI/AMD, DA/DAPI and CMA/DA) treatments were applied to the chromosomes of four Microtidae (Rodentia) species. The results complete the data obtained in our previous paper (Burgos, M., Jiménez, R., & Dìaz de la Guardia, R., Genome 30:540-546, 1988) and prove that the changes in the constitutive heterochromatin in the evolution of the karyotypes of these species are not only due to gain or loss of heterochromatin, but are qualitative with respect to their nucleotide composition, repeated base pair organization or DNA-protein complex modification. These variations lead to the differential response to the fluorescence dye combinations used.
对四种田鼠科（啮齿目）物种的染色体进行了三种不同的荧光染料和特异性复染剂组合（DAPI/AMD、DA/DAPI和CMA/DA）处理。结果完善了我们之前论文（布尔戈斯，M.，希门尼斯，R.，& 迪亚斯·德拉瓜迪亚，R.，《基因组》30:540 - 546，1988）中获得的数据，并证明这些物种核型进化过程中组成型异染色质的变化不仅是由于异染色质的增减，而且在核苷酸组成、重复碱基对组织或DNA - 蛋白质复合物修饰方面存在质的差异。这些变化导致了对所用荧光染料组合的不同反应。
