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用放线菌素A3/偏端霉素A/4',6-二脒基-2-苯基吲哚(DAPI)和D 287/170对猪异染色质进行差异荧光染色。

Differential fluorescent staining of porcine heterochromatin by chromomycin A3/distamycin A/DAPI and D 287/170.

作者信息

Schnedl W, Abraham R, Förster M, Schweizer D

出版信息

Cytogenet Cell Genet. 1981;31(4):249-53. doi: 10.1159/000131664.

DOI:10.1159/000131664
PMID:7341070
Abstract

R banding of porcine chromosomes by chromomycin A3 plus distamycin A and DAPI (DA-DAPI) revealed two distinct types of heterochromatin: The GC-rich centromeric heterochromatin of the biarmed autosomes (Nos. 1-12) and of the X chromosome exhibited bright chromomycin A3 fluorescence, while the heterochromatin of the acrocentric chromosomes (Nos. 13-18) stained brightly by DA-DAPI. The latter also fluoresced brightly when stained by D 287/170. In addition, significant fluorescent polymorphism was observed among both acrocentric and metacentric chromosomes.

摘要

用放线菌素A3加偏端霉素A和4,6-二脒基-2-苯基吲哚(DA-DAPI)对猪染色体进行R显带,揭示了两种不同类型的异染色质:双臂常染色体(1-12号)和X染色体富含GC的着丝粒异染色质呈现明亮的放线菌素A3荧光,而近端着丝粒染色体(13-18号)的异染色质被DA-DAPI染成亮色。后者用D 287/170染色时也发出明亮荧光。此外,在近端着丝粒染色体和中着丝粒染色体中均观察到显著的荧光多态性。

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