Department of Biomedical Analysis, Utrecht University, TB Utrecht, The Netherlands.
Electrophoresis. 2011 Jan;32(1):66-82. doi: 10.1002/elps.201000364. Epub 2010 Nov 30.
CE coupled to MS has proven to be a powerful analytical tool for the characterization of intact proteins, as it combines the high separation efficiency of CE with the selectivity of MS. This review provides an overview of the development and application of CE-MS methods within the field of intact protein analysis as published between January 2007 and June 2010. Ongoing technological developments with respect to CE-MS interfacing, capillary coatings for CE-MS, coupling of CIEF with MS and chip-based CE-MS are treated. Furthermore, CE-MS of intact proteins involving ESI, MALDI and ICP ionization is outlined and overviews of the use of the various CE-MS methods are provided by tables. Representative examples illustrate the applicability of CE-MS for the characterization of proteins, including glycoproteins, biopharmaceuticals, protein-ligand complexes, biomarkers and dietary proteins. It is concluded that CE-MS is a valuable technique with high potential for intact protein analysis, providing useful information on protein identity and purity, including modifications and degradation products.
CE 与 MS 的联用已被证明是一种强大的分析工具，可用于完整蛋白质的特征分析，因为它结合了 CE 的高分离效率和 MS 的选择性。本综述概述了 2007 年 1 月至 2010 年 6 月期间发表的在完整蛋白质分析领域中 CE-MS 方法的发展和应用。文中还讨论了 CE-MS 接口、CE-MS 用毛细管涂层、CIEF 与 MS 的联用和基于芯片的 CE-MS 的最新技术进展。此外，还概述了涉及 ESI、MALDI 和 ICP 电离的完整蛋白质的 CE-MS，并通过表格提供了各种 CE-MS 方法的使用概述。代表性实例说明了 CE-MS 用于蛋白质（包括糖蛋白、生物制药、蛋白质-配体复合物、生物标志物和膳食蛋白质）特征分析的适用性。结论认为，CE-MS 是一种具有高潜力的完整蛋白质分析技术，可为蛋白质的身份和纯度提供有用的信息，包括修饰和降解产物。
