Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2300 RC, Leiden, The Netherlands.
J Proteomics. 2012 Jul 16;75(13):3814-28. doi: 10.1016/j.jprot.2012.04.050. Epub 2012 May 17.
Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful technique for the analysis of proteins and peptides. Over the past few years, significant progress has been made in the development of novel and more effective interfaces for hyphenating CE to MS. This review provides an overview of these new interfacing techniques for coupling CE to MS, covering the scientific literature from January 2007 to December 2011. The potential of these new CE-MS interfacing techniques is demonstrated within the field of (clinical) proteomics, more specifically "bottom-up" proteomics, by showing examples of the analysis of various biological samples. The relevant papers on CE-MS for proteomics are comprehensively summarized in tables, including, e.g. information on sample type and pretreatment, interfacing and MS detection mode. Finally, general conclusions and future perspectives are provided.
毛细管电泳-质谱联用 (CE-MS) 已成为分析蛋白质和肽的强大技术。在过去的几年中，在开发新型和更有效的接口方面取得了重大进展，将 CE 与 MS 连接起来。本综述概述了这些用于将 CE 与 MS 耦合的新型接口技术，涵盖了 2007 年 1 月至 2011 年 12 月的科学文献。通过展示各种生物样品分析的实例，在（临床）蛋白质组学领域，特别是“自上而下”的蛋白质组学领域，展示了这些新型 CE-MS 接口技术的潜力。有关蛋白质组学的 CE-MS 的相关论文在表格中进行了全面总结，包括例如样本类型和预处理、接口和 MS 检测模式的信息。最后，提供了一般结论和未来展望。
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