Department of Chemistry, Isfahan University of Technology, Isfahan, Iran.
J Sep Sci. 2011 Jan;34(1):107-12. doi: 10.1002/jssc.201000583. Epub 2010 Dec 3.
Analysis of testosterone was accomplished using corona discharge ion mobility spectrometry. Molecular imprinted polymer was used for the extraction and pre-concentration of testosterone. Analytical parameters including precision, dynamic range and detection limit were obtained. The linear dynamic range was from 10 to 250 ng/mL and the limit of detection was 0.9 ng/mL. The proposed method was used for analysis of testosterone in urine samples. A urine sample from a 3-year-old girl was used as the blank. The RSD was below 10%. The obtained results from the method were also compared with the standard method for analysis of testosterone using SPE-HPLC analysis. The results demonstrate the accuracy of the method.
采用电晕放电离子淌度谱法对睾酮进行分析。采用分子印迹聚合物对睾酮进行提取和预浓缩。得到了分析参数，包括精密度、线性范围和检测限。线性动态范围为 10 至 250ng/mL，检测限为 0.9ng/mL。该方法用于尿液样品中睾酮的分析。以 3 岁女孩的尿液样品作为空白。RSD 低于 10%。该方法的结果与使用 SPE-HPLC 分析睾酮的标准方法进行了比较。结果表明该方法准确可靠。
