Liu Yongjun, Wu Yinliang, Jiang Yanbin
China Animal Disease Control Center, Beijing 100125, China.
Se Pu. 2010 Sep;28(9):905-7.
A method for the determination of robenidine residue in chicken tissues and eggs by high performance liquid chromatography (HPLC) was established. The samples were extracted with acetonitrile and purified by an HLB solid-phase extraction (SPE) cartridge. The extract was analyzed by HPLC with acetonitrile-0.05 mol/L NH4H2PO4 buffer (6:4, v/v, pH 6.5) as the mobile phase. The flow rate of the mobile phase was 1.0 mL/min and detection wavelength was 317 nm. There was a good linear correlation between the peak areas and the concentrations of robenidine in the range of 10-1000 microg/L. The limit of detection (S/N = 3) was 10 microg/L, and the limit of quantification (S/N = 10) was 15 microg/kg. The recoveries of robenidine were 73.1%-88.7% at the spiked levels of 15, 50 and 100 microg/kg. The results demonstrate that the method is easy, fast, sensitive, and suitable for the confirmation and quantification of robenidine residue in chicken tissues and eggs. The clean-up effect, sensitivity, accuracy and precision can all meet the analysis requirement.
建立了一种用高效液相色谱法（HPLC）测定鸡肉组织和鸡蛋中罗苯哒唑残留量的方法。样品用乙腈提取，并用HLB固相萃取（SPE）柱净化。提取物用乙腈-0.05 mol/L磷酸二氢铵缓冲液（6:4，v/v，pH 6.5）作为流动相进行HPLC分析。流动相流速为1.0 mL/min，检测波长为317 nm。罗苯哒唑的峰面积与浓度在10 - 1000 μg/L范围内呈良好的线性关系。检测限（S/N = 3）为10 μg/L，定量限（S/N = 10）为15 μg/kg。在15、50和100 μg/kg的加标水平下，罗苯哒唑的回收率为73.1% - 88.7%。结果表明，该方法简便、快速、灵敏，适用于鸡肉组织和鸡蛋中罗苯哒唑残留量的确证和定量分析。净化效果、灵敏度、准确度和精密度均能满足分析要求。
