Gao Feng, Zhou Su-ya, Xia Qiang, Luo Jian-hong
College of Medicine, Zhejiang University, Department of Neurobiology, Hangzhou 310031, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 May;21(2):156-9.
To investigate the cardiovascular response caused by intracerebroventricular (ICV) microinjection of interleukin-2 (IL-2) and explore the underlying mechanism.
Male Sprague-Dawley rats were anesthetized with intraperitoneal urethane( 1.2 g/ kg). The changes of mean arterial blood pressure (MAP) and heart rate (HR) were observed during ICV microinjection of IL-2 with or without pretreatment of naloxone or atropine or phentolamine.
There were no significant effects on cardiovascular response after ICV injection of IL-2 at 500 IU/3 microl and 1 000 IU/3 microl, but IL-2 at 1 500 IU/3 microl could elevate MAP and HR. The responses of MAP and HR reached their maximum levels at 10 min (MAP: 10 +/- 1.8 mmHg, HR: 25 +/- 2 b/min, P < 0.05) after the injection and lasted 15 or 10 minutes respectively. Pretreatment with naloxone (10 microg/10 microl) or atropine (1.5 microg/10 microl) could block the cardiovascular response of ICV injection of IL-2. Pretreatment with phentolamine (10 microg/10 microl) failed to block the cardiovascular responses by IL-2.
ICV microinjection of interleukin-2 (IL-2) can elevate the MAP and HR, which may be mediated by central opioid and cholinergic system. The alpha-adrenergic system may be not involved in the cardiovascular response of IL-2.
研究脑室内（ICV）微量注射白细胞介素-2（IL-2）引起的心血管反应，并探讨其潜在机制。
雄性Sprague-Dawley大鼠腹腔注射氨基甲酸乙酯（1.2 g/kg）麻醉。在ICV微量注射IL-2时，观察有无纳洛酮、阿托品或酚妥拉明预处理情况下平均动脉血压（MAP）和心率（HR）的变化。
ICV注射500 IU/3 μl和1000 IU/3 μl的IL-2后对心血管反应无显著影响，但1500 IU/3 μl的IL-2可升高MAP和HR。注射后10分钟时MAP和HR反应达到最高水平（MAP：10±1.8 mmHg，HR：25±2次/分钟，P<0.05），分别持续15分钟或10分钟。纳洛酮（10 μg/10 μl）或阿托品（1.5 μg/10 μl）预处理可阻断ICV注射IL-2的心血管反应。酚妥拉明（10 μg/10 μl）预处理未能阻断IL-2引起的心血管反应。
ICV微量注射白细胞介素-2（IL-2）可升高MAP和HR，这可能由中枢阿片类和胆碱能系统介导。α-肾上腺素能系统可能不参与IL-2的心血管反应。
