Wang Wei-Ping, Lou Yan, Li Zhen-Zhong, Li Pan, Duan Rui-Sheng
Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Feb;23(1):51-5.
SD rats were utilized for the purpose of the exploration of effects of status epilepticus (SE) on their emotional behavior, spatial learning and memory, and explorating its molecular mechanism.
Forty maturity male SD rats, weighing (200 +/- 20) g were divided randomly and equally into SE group (SG) and normal control group (NG). The SG rats were induced by Pentylenetetrazole (PTZ) and the control animals received a saline (0.9%) solution. The change of emotional behavior in two groups were tested in elevated plus maze. Furthermore, Morris water maze was applied to evaluate the effects by SE on spatial learning and memory in rats. At the same time, N-methyl-D-aspartate (NMDA) receptor NR1 subunit mRNA in the hippocampus was determined by reverse transcription polymerase chain reaction (RT-PCR).
In elevated plus test, SE rats increased the times of visits as well as the time spent on the open arms of the elevated plus maze (P < 0.01). In Morris water maze, the mean escape latency for the SE rats looking for hidden platform in the place navigation test prolonged (P < 0.01). The efficiency of their search strategy was poor (P < 0.05). The swimming time in platform region and the percentage of their swimming time decreased (P < 0.01). The number of times they crossed the platform area decreased (P < 0.01). Meanwhile the expression of NR1 subunit mRNA in hippocampus was lower (P < 0.01).
The experimental results showed that SE could result in the change of emotional behavior and damage of spatial learning and memory in rats. NR1 might be involved in the patho- and physiological process in causing these behavioral changes.
利用SD大鼠探索癫痫持续状态（SE）对其情绪行为、空间学习和记忆的影响，并探讨其分子机制。
将40只体重（200±20）g的成年雄性SD大鼠随机等分为癫痫持续状态组（SG）和正常对照组（NG）。SG组大鼠用戊四氮（PTZ）诱导，对照组动物给予生理盐水（0.9%）溶液。在高架十字迷宫中测试两组的情绪行为变化。此外，应用Morris水迷宫评估SE对大鼠空间学习和记忆的影响。同时，采用逆转录聚合酶链反应（RT-PCR）检测海马中N-甲基-D-天冬氨酸（NMDA）受体NR1亚基mRNA。
在高架十字试验中，SE大鼠增加了在高架十字迷宫开放臂的访问次数和停留时间（P<0.01）。在Morris水迷宫中，SE大鼠在位置导航试验中寻找隐藏平台的平均逃避潜伏期延长（P<0.01）。其搜索策略的效率较差（P<0.05）。在平台区域的游泳时间及其游泳时间百分比下降（P<0.01）。它们穿过平台区域的次数减少（P<0.01）。同时海马中NR1亚基mRNA的表达较低（P<0.01）。
实验结果表明，SE可导致大鼠情绪行为改变和空间学习记忆损伤。NR1可能参与了导致这些行为变化的病理生理过程。
