[体外心肌缺血/再灌注对兔心肌细胞核膜NTP酶活性及mRNA输出的影响]
[Influence of myocardial ischemia/reperfusion in vitro on nuclear envelope NTPase activity and mRNA export in the cardiomyocyte of rabbits].
作者信息
Xia C F, Liu N K, Yang J, Pang Y Z, Huo Y, Tang C S
机构信息
Institute of Cardiovascular Research, The First Hospital, Peking University, Beijing 100034, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2001 May;17(2):161-5.
AIM
To study the alteration of nuclear envelope associated NTPase activity and mRNA nucleocytoplasmic transport in rabbit cardiomyocyte following in vitro ischemia and reperfusion.
METHODS
The model of myocardial perfusion in rabbit was used to produce myocardial I/R injury and cardiomyocyte nuclear envelope vesicles were prepared by density gradient centrifugation for the assay of mRNA transport rate and NTPase activity.
RESULTS
NTPase activity was reduced and mRNA transport rate was significantly decreased in I/R (P < 0.01) but not in ischemia group( P > 0.05).
CONCLUSION
The nuclear envelope vesicles had been injured following ischemia and reperfusion and resulted in NTPase activity reduced and egress of mRNA interrupted, and therefore may lead to decreasing of protein synthesis.
目的
研究体外缺血再灌注后兔心肌细胞核膜相关NTP酶活性及mRNA核质转运的变化。
方法
采用兔心肌灌注模型制备心肌缺血/再灌注损伤，通过密度梯度离心法制备心肌细胞核膜囊泡，用于检测mRNA转运速率和NTP酶活性。
结果
缺血/再灌注组NTP酶活性降低，mRNA转运速率显著下降（P<0.01），而缺血组无明显变化（P>0.05）。
结论
缺血再灌注后核膜囊泡受损，导致NTP酶活性降低，mRNA输出中断，进而可能导致蛋白质合成减少。
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