雅培实时 HIV-1 m2000rt 病毒载量检测：手动提取与自动化 m2000sp 提取。
Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction.
机构信息
University of the Witwatersrand, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, 7 York Rd Parktown, Johannesburg 2000, South Africa.
出版信息
J Virol Methods. 2011 Mar;172(1-2):78-80. doi: 10.1016/j.jviromet.2010.12.006. Epub 2010 Dec 21.
Abstract
The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually.
摘要
雅培实时 HIV-1 检测法是一种实时核酸扩增检测法，可用于 HIV-1 病毒载量定量。该检测法具有从血浆或干血斑样本中自动提取病毒 RNA 的平台，以及具有实时荧光检测的扩增平台。总的来说，如果样本是手动提取的，本研究未发现病毒载量存在临床相关差异。
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