枯草芽孢杆菌中分解代谢物阻遏操纵序列的定点诱变
Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.
作者信息
Weickert M J, Chambliss G H
机构信息
Department of Genetics, University of Wisconsin, Madison 53706.
出版信息
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6238-42. doi: 10.1073/pnas.87.16.6238.
Abstract
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression.
摘要
枯草芽孢杆菌α-淀粉酶基因（amyE）的分解代谢物阻遏涉及一个位于启动子（amyR）下游紧邻处、与转录起始位点重叠的操纵序列。对该序列进行寡核苷酸定点诱变确定了分解代谢物阻遏所需的碱基。两个突变增加了操纵子的2倍对称性以及阻遏率。尽管许多突变使阻遏率降低了3至11倍，但有些突变也使淀粉酶产量提高了2倍或更多。其他突变导致超量产生但不影响分解代谢物阻遏。其他受分解代谢物阻遏的枯草芽孢杆菌启动子中的同源序列表明，一个共同的调控位点可能参与了分解代谢物阻遏。
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