State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Haidian District, Beijing 100093, PR China.
Plant Cell Physiol. 2011 Feb;52(2):306-16. doi: 10.1093/pcp/pcq186. Epub 2010 Dec 20.
Depletion of stratospheric ozone has led to increased UV radiation reaching the surface of the Earth. This may damage plants. Using physiological, proteomic and quantitative real-time PCR (qPCR) methods, we systematically studied the response of 16-day-old rice seedlings to UV [0.67 W m(-2) biologically effective UVB (UVB(BE)) and 0.28 W m(-2) UVA] exposure for 6, 12 and 24 h. UV exposure resulted in the appearance of light brown patches on leaves, a decrease in the net photosynthetic rate (Pn), lipid peroxidation, accumulation of UV-absorbing compounds (including flavonoids and other phenolic pigments) and differential expression of 22 proteins. Both physiological and molecular responses became stronger with increasing UV exposure time, indicating the effects of UV accumulation on plants. UV-induced responses included (i) phytohormone-regulative responses (up-regulation of proteins related to phytohormone synthesis such as IAA and ethylene); (ii) injurious responses (photosynthesis suppression, lipid peroxidation and visible injury); and (iii) protective responses (accumulation of UV-absorbing compounds and differential expression of proteins involved in detoxification/antioxidation, defense, protein processing, RNA processing, carbohydrate metabolism and secondary metabolism). The identification of UV-responsive proteins provided a better understanding of the molecular mechanism of plant responses to UV stress. Proteomic and qPCR analysis identified one up-regulated and two induced proteins with important functions: tryptophan synthase α chain (production of radical oxygen species), glyoxalase I (detoxification/antioxidation) and a Bet v I family protein (defense). These results will contribute to future research into their roles in UV stress responses in plants.
平流层臭氧的消耗导致到达地球表面的紫外线辐射增加。这可能会损害植物。我们使用生理、蛋白质组学和定量实时 PCR（qPCR）方法，系统地研究了 16 天大的水稻幼苗对 UV [0.67 W m(-2) 生物有效 UVB（UVB(BE)）和 0.28 W m(-2) UVA]暴露 6、12 和 24 h 的反应。UV 暴露导致叶片出现浅棕色斑块，净光合速率（Pn）下降，脂质过氧化，紫外线吸收化合物（包括类黄酮和其他酚类色素）积累和 22 种蛋白质的差异表达。随着 UV 暴露时间的增加，生理和分子反应都变得更强，这表明 UV 积累对植物的影响。UV 诱导的反应包括（i）植物激素调节反应（上调与植物激素合成相关的蛋白质，如 IAA 和乙烯）；（ii）伤害反应（光合作用抑制、脂质过氧化和可见损伤）；和（iii）保护反应（紫外线吸收化合物的积累和参与解毒/抗氧化、防御、蛋白质加工、RNA 加工、碳水化合物代谢和次生代谢的蛋白质的差异表达）。UV 响应蛋白的鉴定提供了对植物对 UV 胁迫反应的分子机制的更好理解。蛋白质组学和 qPCR 分析鉴定了一个上调和两个具有重要功能的诱导蛋白：色氨酸合酶α链（产生活性氧）、醛糖还原酶 I（解毒/抗氧化）和 Bet v I 家族蛋白（防御）。这些结果将有助于未来研究它们在植物对 UV 胁迫反应中的作用。
