Japan Bioassay Research Center, Japan Industrial Safety and Health Association, 2445 Hirasawa, Hadano, Kanagawa 257-0015, Japan.
Ind Health. 2011;49(2):215-20. doi: 10.2486/indhealth.ms1213. Epub 2010 Dec 16.
In order to assess the extrapulmonary effects of multiwall carbon nanotubes (MWCNT), deposition of MWCNT and histopathologic changes in lung-associated lymph nodes (LALN) were examined in MWCNT-administered rats. At the age of 13 wk, male F344 rats were intratracheally instilled with MWCNT at a dose of 0 (vehicle), 40 or 160 μg/rat. The rats were sacrificed on Day 1, 7, 28 or 91 after instillation and light microscopic examinations were performed on LALN tissues. MWCNT was translocated to right and left posterior mediastinal lymph nodes and parathymic lymph nodes. Deposition of MWCNT was greater in the posterior mediastinal lymph node than in the parathymic lymph node, and the amount of MWCNT deposited in these two lymph nodes increased gradually and dose-dependently with time. MWCNT was phagocytosed by nodal macrophages, and some of the MWCNT-laden macrophages were aggregated. Transmission electron microscopic (TEM) observation confirmed the presence of MWCNT fibers with a characteristic multi-walled cylindrical structure.
为了评估多壁碳纳米管（MWCNT）的肺外效应，研究了 MWCNT 给药大鼠肺相关淋巴结（LALN）中的 MWCNT 沉积和组织病理学变化。在 13 周龄时，雄性 F344 大鼠经气管内滴注给予 0（载体）、40 或 160μg/大鼠剂量的 MWCNT。在滴注后第 1、7、28 或 91 天处死大鼠，并对 LALN 组织进行光镜检查。MWCNT 转移到右后纵隔淋巴结和甲状旁腺淋巴结。MWCNT 在纵隔后淋巴结中的沉积量大于甲状旁腺淋巴结，并且这两个淋巴结中沉积的 MWCNT 量随时间逐渐增加且呈剂量依赖性。MWCNT 被淋巴结巨噬细胞吞噬，一些载有 MWCNT 的巨噬细胞聚集在一起。透射电子显微镜（TEM）观察证实存在具有特征性多壁圆柱形结构的 MWCNT 纤维。
