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本文引用的文献

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In situ enzymatic screening (ISES) of P,N-ligands for Ni(0)-mediated asymmetric intramolecular allylic amination.用于镍（0）介导的不对称分子内烯丙基胺化反应的P，N配体的原位酶筛选（ISES）
Tetrahedron Asymmetry. 2004 Sep 20;15(18):2845-2851. doi: 10.1016/j.tetasy.2004.06.052. Epub 2004 Sep 13.
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Chemoenzymatic synthesis of differentially protected 3-deoxysugars.酶促化学合成差向保护的 3-脱氧糖。
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Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids.苏氨酸醛缩酶的筛选、性质及其在非蛋白源β-羟基-α-氨基酸合成中的应用。
Appl Microbiol Biotechnol. 2010 Sep;88(2):409-24. doi: 10.1007/s00253-010-2751-8. Epub 2010 Aug 4.
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Biocatalytic asymmetric synthesis of chiral amines from ketones applied to sitagliptin manufacture.手性胺的生物催化不对称合成从酮应用于西他列汀的制造。
Science. 2010 Jul 16;329(5989):305-9. doi: 10.1126/science.1188934. Epub 2010 Jun 17.
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Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.迭代饱和突变加速酶立体选择性的实验室进化：与传统方法的严格比较。
J Am Chem Soc. 2010 Jul 7;132(26):9144-52. doi: 10.1021/ja1030479.
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Exploiting the regioselectivity of Baeyer-Villiger monooxygenases for the formation of beta-amino acids and beta-amino alcohols.利用拜耳-维利格单加氧酶的区域选择性来合成β-氨基酸和β-氨基醇。
Angew Chem Int Ed Engl. 2010 Jun 14;49(26):4506-8. doi: 10.1002/anie.201000511.
7
Use of a robust dehydrogenase from an archael hyperthermophile in asymmetric catalysis-dynamic reductive kinetic resolution entry into (S)-profens.利用一种来自古嗜热菌的强脱氢酶进行不对称催化——动态还原动力学拆分法进入（S）-普仑司特。
J Am Chem Soc. 2010 May 5;132(17):5930-1. doi: 10.1021/ja910778p.
8
Reconstructing the clostridial n-butanol metabolic pathway in Lactobacillus brevis.在短乳杆菌中重建梭菌丁酸代谢途径。
Appl Microbiol Biotechnol. 2010 Jun;87(2):635-46. doi: 10.1007/s00253-010-2480-z. Epub 2010 Mar 2.
9
Enantioselective biocatalytic oxidative desymmetrization of substituted pyrrolidines.取代吡咯烷的对映选择性生物催化氧化去对称化反应
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10
Chemoenzymatic synthesis of alpha-hydroxy-beta-methyl-gamma-hydroxy esters: role of the keto-enol equilibrium to control the stereoselective hydrogenation in a key step.酶促化学合成α-羟基-β-甲基-γ-羟基酯：酮-烯醇平衡在关键步骤中控制立体选择性氢化的作用。
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梭菌中用于不对称合成的一种新的脱氢酶：动态还原动力学拆分进入 Taxotère 侧链。

A new dehydrogenase from Clostridium acetobutylicum for asymmetric synthesis: dynamic reductive kinetic resolution entry into the Taxotère side chain.

机构信息

Department of Chemistry & Nebraska Center for Energy Sciences Research, University of Nebraska, Lincoln, NE, USA.

出版信息

Chem Commun (Camb). 2011 Feb 28;47(8):2420-2. doi: 10.1039/c0cc04585c. Epub 2010 Dec 20.

DOI:10.1039/c0cc04585c
PMID:21173953
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4882475/
Abstract

An NADP-dependent alcohol dehydrogenase from Clostridium acetobutylicum (CaADH) has been expressed and characterized. CaADH enantioselectively reduces aromatic α-, β- and γ-keto esters to the corresponding D-hydroxy esters and provides a building block for the Taxotère side chain (95% yield, 95% de, 99% ee) by dynamic reductive kinetic resolution (DYRKR).

摘要

已表达和表征了来自丙酮丁醇梭菌（CaADH）的一种依赖 NADP 的醇脱氢酶。CaADH 对映选择性地将芳香族 α-、β-和 γ-酮酯还原为相应的 D-羟基酯，并通过动态还原动力学拆分（DYRKR）为 Taxotère 侧链提供构建块（95%产率、95%de、99%ee）。