一种用于测定马红细胞碳酸酐酶（CA）和过氧化氢酶（Cat）基因变体的单一凝胶。
A single gel for determining genetic variants of equine erythrocyte carbonic anhydrase (CA) and catalase (Cat).
作者信息
Bowling A T, Gordon L, Penedo M C, Wictum E, Beebout J
机构信息
Serology Laboratory, School of Veterinary Medicine, University of California, Davis.
出版信息
Anim Genet. 1990;21(2):191-7. doi: 10.1111/j.1365-2052.1990.tb03224.x.
We describe a method for agarose IEF under acid conditions in which a single gel can be used to diagnose from equine red cell lysates genetic variants for carbonic anhydrase (CA) and catalase (Cat). Family and population data for 4801 horses of 27 breeds and seven trap sites of Great Basin feral horses are presented to support the presence of a sixth CA allele, CAE, which has been recognized previously, but not described by published data. Allelic frequencies for the two systems suggest it may be appropriate to use this gel for parentage verification programmes or to obtain population data for studies of the genus Equus.
我们描述了一种在酸性条件下进行琼脂糖等电聚焦的方法，其中单个凝胶可用于从马红细胞裂解物中诊断碳酸酐酶（CA）和过氧化氢酶（Cat）的遗传变异。展示了来自27个品种的4801匹马以及大盆地野马的七个捕获地点的家族和种群数据，以支持第六个CA等位基因CAE的存在，该等位基因先前已被识别，但尚未有公开数据描述。这两个系统的等位基因频率表明，使用这种凝胶进行亲子鉴定程序或获取马属研究的种群数据可能是合适的。
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