Department of Pathology, Chinese People's Liberation Army (PLA) General Hospital, 28 Fuxing Road, Beijing 100853, China.
Pathol Oncol Res. 2011 Jun;17(2):369-73. doi: 10.1007/s12253-010-9335-x. Epub 2010 Dec 21.
The relationships between the expression of cyclin B1, EBV-LMP1, P53 and P16(INK4A) in Chinese classical Hodgkin lymphoma are unknown and need exploring. Samples of classical Hodgkin lymphoma from 60 Chinese patients were analyzed for the expression of cyclin B1, EBV-LMP1, P53 and P16(INK4A) proteins by immunohistochemistry. Cyclin B1 protein was overexpressed in 90.0% (54/60) of this group of classical Hodgkin lymphoma, staining mainly and strongly in cytoplasm but also sparsely and weakly in nucleus of the Hodgkin and Reed-Sternberg (HRS) cells. EBV-LMP1, P53 and P16(INK4A) were overexpressed in 85.0%, 96.7% and 71.7% of Hodgkin lymphoma, respectively. EBV-LMP1, P53 and P16(INK4A) were was noted in the nucleus of HRS cells. Microscopically, cyclin B1 and P53 staining distinguished the HRS cells from the complex background of lymphocytes. Cyclin B1 was positively correlated with EBV-LMP1(P < 0.001) and P53(P < 0.001), but was inversely related to P16(INK4A) (P < 0.05). It is suggested that overexpression of cyclin B1 could play an important role in the evolution of classical Hodgkin lymphoma, and cyclin B1 may be considered as a potential adjunct marker to identify HRS cells in diagnosis and be served as Hodgkin lymphoma-associated antigen in the near future.
在中国经典型霍奇金淋巴瘤中，细胞周期蛋白 B1、EBV-LMP1、P53 和 P16(INK4A) 的表达之间的关系尚不清楚，需要进一步研究。通过免疫组织化学方法分析了 60 例中国经典型霍奇金淋巴瘤患者样本中细胞周期蛋白 B1、EBV-LMP1、P53 和 P16(INK4A)蛋白的表达。在这组经典型霍奇金淋巴瘤中，90.0%（54/60）的细胞周期蛋白 B1 蛋白过度表达，主要和强烈染色于细胞质，但也稀疏和弱染色于霍奇金和里德-斯特恩伯格（HRS）细胞的核内。EBV-LMP1、P53 和 P16(INK4A)在霍奇金淋巴瘤中的过度表达率分别为 85.0%、96.7%和 71.7%。EBV-LMP1、P53 和 P16(INK4A)均染色于 HRS 细胞的核内。显微镜下，细胞周期蛋白 B1 和 P53 的染色可将 HRS 细胞与淋巴细胞的复杂背景区分开来。细胞周期蛋白 B1 与 EBV-LMP1（P<0.001）和 P53（P<0.001）呈正相关，但与 P16(INK4A)呈负相关（P<0.05）。这表明细胞周期蛋白 B1 的过度表达可能在经典型霍奇金淋巴瘤的发生中起重要作用，细胞周期蛋白 B1 可作为一种潜在的辅助标志物，有助于在诊断中识别 HRS 细胞，并在不久的将来作为霍奇金淋巴瘤相关抗原。
