Smola T, Specht G, Liebermann H
VEB Friedrich-Loeffler-Institut, Insel Riems, Betrieb des VEB Kombinat Veterinärimpfstoffe Dessau.
Arch Exp Veterinarmed. 1990;44(2):311-7.
Described in this paper is a physical method by which to determine virus concentrations, including ultracentrifugation of a sample through a saccharose gradient and subsequent analysis of tubule content by means of a flow photometer. Here before, the curves thus obtained used to be graphically evaluated by means of stencil plates. Direct recording and processing of the extinction-time curve by means of a KC 85/1 minicomputer have substantially improved accuracy of results. Time consumption has been reduced, and reading as well as calculating errors have been entirely ruled out. Repetition of determinations confirmed that virus concentrations could be determined at an accuracy of four to seven percent.
本文描述了一种测定病毒浓度的物理方法,包括通过蔗糖梯度对样品进行超速离心,并随后借助流动光度计分析小管内容物。在此之前,由此获得的曲线过去是通过模板进行图形评估的。借助KC 85/1微型计算机对消光时间曲线进行直接记录和处理,大大提高了结果的准确性。时间消耗减少了,读数和计算误差也完全消除了。重复测定证实,病毒浓度的测定精度可达4%至7%。