Xu Gang, Hou Chen-Rui, Jiang Hao-Wen, Xiang Cui-Qin, Shi Nong, Yuan Hai-Chuan, Ding Qiang, Zhang Yuan-Fang
Department of Urology, Huashan Hospital, Fu Dan University, China.
Indian J Biochem Biophys. 2010 Aug;47(4):211-8.
Diagnostic biomarkers for early detection of renal cell carcinoma (RCC) are in great need. In the present study, we compared the serum protein profiles of patients with small RCC to those of healthy individuals to identify the differentially expressed proteins with potential to serve as biomarkers. Serum samples were collected from 10 patients with small RCC and 10 healthy individuals. The serum protein expression profiles were analyzed by two-dimensional (2-D) gel electrophoresis. Twenty-seven proteins with differences in expression levels between RCC patients and healthy volunteers were identified. Of these, 19 were expressed at different levels and eight were expressed in serum from the RCC group, but not from the control group. Six differentially expressed proteins identified by using mass spectrometry included coagulation factor XIII B, complement C3 and its precursor, misato homolog 1 (isoform CRA_b), hemopexin, and alpha-1-B-glycoprotein. Some of these serum proteins are known regulators of tumor progression in human malignancies. In conclusion, we successfully applied 2-D gel electrophoresis and identified six serum proteins differentially expressed between patients with small RCC and healthy volunteers. These proteins may provide novel biomarkers for early detection and diagnosis of human RCC.
目前迫切需要用于早期检测肾细胞癌（RCC）的诊断生物标志物。在本研究中，我们比较了小肾癌患者与健康个体的血清蛋白质谱，以鉴定有潜力作为生物标志物的差异表达蛋白。收集了10例小肾癌患者和10名健康个体的血清样本。通过二维（2-D）凝胶电泳分析血清蛋白质表达谱。鉴定出27种在RCC患者和健康志愿者之间表达水平存在差异的蛋白质。其中，19种在不同水平表达，8种在RCC组血清中表达，但在对照组血清中未表达。通过质谱鉴定出的6种差异表达蛋白包括凝血因子XIII B、补体C3及其前体、Misato同源物1（异构体CRA_b）、血红素结合蛋白和α-1-B-糖蛋白。这些血清蛋白中的一些是人类恶性肿瘤中已知的肿瘤进展调节因子。总之，我们成功应用二维凝胶电泳并鉴定出6种在小肾癌患者和健康志愿者之间差异表达的血清蛋白。这些蛋白质可能为人类RCC的早期检测和诊断提供新的生物标志物。
