School of Resource and Environmental Engineering, Heilongjiang Institute of Science and Technology, Harbin 150027, China.
J Environ Sci (China). 2010;22(9):1312-8. doi: 10.1016/s1001-0742(09)60256-4.
Aerobic granular sludge was cultivated by using different kinds of seed sludge in sequencing batch airlift reactor. The influence of seed sludge on physical and chemical properties of granular sludge was studied; the microbial community structure was probed by using scanning electron microscope and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that seed sludge played an important role on the formation of aerobic granules. Seed sludge taken from beer wastewater treatment plant (inoculum A) was more suitable for cultivating aerobic granules than that of sludge from municipal wastewater treatment plant (inoculum B). Cultivated with inoculum A, large amount of mature granules formed after 35 days operation, its SVI reached 32.75 mL/g, and SOUR of granular sludge was beyond 1.10 mg/(g x min). By contrast, it needed 56 days obtaining mature granules using inoculum B. DGGE profiles indicated that the dominant microbial species in mature granules were 18 and 11 OTU when inoculum A and B were respectively employed as seed sludge. The sequencing results suggested that dominant species in mature granules cultivated by inoculum A were Paracoccus sp., Devosia hwasunensi, Pseudoxanthomonas sp., while the dominant species were Lactococcus raffinolactis and Pseudomonas sp. in granules developed from inoculum B.
采用不同种接种污泥在序批式气升环流反应器中培养好氧颗粒污泥。研究了接种污泥对颗粒污泥物理化学性质的影响；采用扫描电子显微镜和聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）探究了微生物群落结构。结果表明，接种污泥对好氧颗粒的形成起着重要作用。与来自城市污水处理厂的污泥（接种物 B）相比，来自啤酒废水处理厂的污泥（接种物 A）更适合培养好氧颗粒。采用接种物 A 培养 35 天后形成大量成熟颗粒，其 SVI 达到 32.75 mL/g，颗粒污泥 SOUR 超过 1.10 mg/（g·min）。相比之下，采用接种物 B 需要 56 天才能获得成熟颗粒。DGGE 图谱表明，当分别使用接种物 A 和 B 作为接种污泥时，成熟颗粒中的优势微生物物种分别为 18 和 11 个 OTU。测序结果表明，接种物 A 培养的成熟颗粒中的优势种为 Paracoccus sp.、Devosia hwasunensi、Pseudoxanthomonas sp.，而由接种物 B 形成的颗粒中的优势种为 Lactococcus raffinolactis 和 Pseudomonas sp.。
