Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
J Environ Sci (China). 2010;22(9):1381-6. doi: 10.1016/s1001-0742(09)60265-5.
Electro-bioremediation is an innovative method to remedy organic-polluted soil. However, the principle of electrokinetic technology enhancing the function of microbes, especially the relationship of electric intensity and biodegradation efficiency, is poorly investigated. Petroleum was employed as a target organic pollutant at a level of 50 g/kg (mass of petroleum/mass of dry soil). A direct current power supply was used for tests with a constant direct current electric voltage (1.0 V/cm). The petroleum concentrations were measured at 3275-3285 nm after extraction using hexane, the group composition of crude oil was analyzed by column chromatography. The water content of soil was kept 25% (m/m). The results indicated the degradation process was divided into two periods: from day 1 to day 40, from day 41 to day 100. The treatment of soil with an appropriate electric field led the bacteria to have a persistent effect in the whole period of 100 days. The highest biodegradation efficiency of 45.5% was obtained after treatment with electric current and bacteria. The electric-bioremediation had a positive effect on alkane degradation. The degradation rate of alkane was 1.6 times higher in the soil exposed to electric current than that treated with bacteria for 100 days. A proper direct current could stimulate the microbial activities and accelerate the biodegradation of petroleum. There was a positive correlation between the electric intensities and the petroleum bioremediation efficiencies with a coefficient of 0.9599.
电生物修复是一种修复有机污染土壤的创新方法。然而，电动力学技术增强微生物功能的原理，尤其是电场强度与生物降解效率的关系，研究得还不够充分。以 50g/kg（石油质量/干土质量）的浓度将石油作为目标有机污染物。使用直流电进行测试，恒流直流电压为 1.0V/cm。用正己烷萃取后，在 3275-3285nm 处测量石油浓度，采用柱层析法分析原油的族组成。土壤的含水量保持在 25%（m/m）。结果表明，降解过程分为两个时期：第 1 天到第 40 天，第 41 天到第 100 天。在适当的电场处理下，土壤中的细菌在 100 天的整个周期中持续发挥作用。经电流和细菌处理后，生物降解效率最高可达 45.5%。电生物修复对烷烃降解有积极影响。在暴露于电流的土壤中，烷烃的降解速率比用细菌处理 100 天的降解速率高 1.6 倍。适当的直流电可以刺激微生物的活性，加速石油的生物降解。电场强度与石油生物修复效率之间存在正相关关系，相关系数为 0.9599。
