Ye Xi-Qiong, Wu Man-Li, Chen Kai-Li, Li Wei, Yuan Jing
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2017 Feb 8;38(2):728-734. doi: 10.13227/j.hjkx.201608008.
A laboratory study was conducted to investigate the impacts of bioremediation on microbial communities and various nitrogen shifts in petroleum contaminated soil by using GC-MS and Illumia MiSeq technique. Results showed the concentrations of alkane reduced from 25987.8 mg·kg to 12788.6 mg·kg, and the concentrations of polycyclic aromatic hydrocarbons (PAHs) decreased from 5322.9 mg·kg to 2917.2 mg·kg. Illumia MiSeq results showed that soil microbial communities shifted significantly after remediation, and the relative abundance of some phylum of hydrocarbon degraders (Firmicutes, Bacterodetes), and some genus of degraders () increased. Besides, the contents of total nitrogen and ammonia nitrogen increased firstly and then decreased during remediation. However, the contents of nitrate nitrogen decreased at the early stage, and then kept stable in the later stage of remediation. It can be concluded that bioremediation effectively promoted petroleum hydrocarbon degradation, and the different fractional hydrocarbon degradation was related to the relative abundance of hydrocarbon degraders and available nitrogen contents.
通过气相色谱-质谱联用仪（GC-MS）和Illumia MiSeq技术进行了一项实验室研究，以调查生物修复对石油污染土壤中微生物群落和各种氮素变化的影响。结果表明，烷烃浓度从25987.8 mg·kg降至12788.6 mg·kg，多环芳烃（PAHs）浓度从5322.9 mg·kg降至2917.2 mg·kg。Illumia MiSeq结果显示，修复后土壤微生物群落发生了显著变化，一些烃降解菌门（厚壁菌门、拟杆菌门）和一些降解菌属（）的相对丰度增加。此外，修复过程中总氮和氨氮含量先增加后降低。然而，硝态氮含量在修复初期降低，在修复后期保持稳定。可以得出结论，生物修复有效地促进了石油烃的降解，不同馏分的烃降解与烃降解菌的相对丰度和有效氮含量有关。
