具有酵母起始tRNA(Met)序列的化学合成RNA的特性分析
Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met).
作者信息
Bratty J, Wu T F, Nicoghosian K, Ogilvie K K, Perreault J P, Keith G, Cedergren R
机构信息
Département de Biochimie, Université de Montréal, Qué, Canada.
出版信息
FEBS Lett. 1990 Aug 20;269(1):60-4. doi: 10.1016/0014-5793(90)81118-8.
A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli.
化学合成了一段与酿酒酵母起始tRNA序列相对应的75个单位长的寡核糖核苷酸。对粗RNA进行了纯化，并通过RNA测序技术验证了序列。一个特别有用的纯化步骤涉及在BND-纤维素上进行疏水层析。纯化后的RNA可被氨酰化至真实起始tRNA（Met）样品的28%，并被苏氨酰化至大肠杆菌天然tRNA（fMet）所观察到水平的76%。
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