酵母甲硫氨酸起始tRNA的T-ψ-茎中磷酸化O-核糖基腺苷的存在。
Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.
作者信息
Desgrès J, Keith G, Kuo K C, Gehrke C W
机构信息
Laboratoire de Biochimie Médicale, Université de Bourgogne, Dijon, France.
出版信息
Nucleic Acids Res. 1989 Feb 11;17(3):865-82. doi: 10.1093/nar/17.3.865.
Abstract
We report in this paper on isolation and characterization of two unknown nucleosides G* and [A*] located in the T-psi-stem of yeast methionine initiator tRNA, using the combined means of HPLC protocols, real time UV-absorption spectrum, and post-run mass spectrometry by electron impact or fast atom bombardment. The G* nucleoside in position 65 was identified as unmodified guanosine. The structure of the unknown [A*] in position 64 was characterized as an isomeric form of O-ribosyl-adenosine by comparison of its chromatographic, UV-spectral and mass spectrometric properties with those of authentic O-alpha-ribofuranosyl-(1"----2')-adenosine isolated from biosynthetic poly(adenosine diphosphate ribose). Our studies also brought evidence for the presence of a phosphorylmonoester group located on this new modified nucleoside [A*], when isolated by ion exchange chromatography from enzymic hydrolysis of yeast initiator tRNAMet without phosphatase treatment.
摘要
在本文中，我们报告了利用高效液相色谱法、实时紫外吸收光谱以及电子轰击或快原子轰击的运行后质谱等综合手段，对酵母甲硫氨酸起始tRNA的T-ψ-茎中两个未知核苷G和[A]进行分离和表征的研究。65位的G核苷被鉴定为未修饰的鸟苷。通过将64位未知[A]的色谱、紫外光谱和质谱性质与从生物合成的聚（二磷酸腺苷核糖）中分离得到的 authentic O-α-呋喃核糖基-(1"----2')-腺苷的性质进行比较，其结构被表征为O-核糖基腺苷的异构体形式。我们的研究还提供了证据，表明当通过离子交换色谱法从未经磷酸酶处理的酵母起始tRNAMet的酶促水解产物中分离出这种新的修饰核苷[A*]时，该核苷上存在一个磷酸单酯基团。
相似文献
1
Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.酵母甲硫氨酸起始tRNA的T-ψ-茎中磷酸化O-核糖基腺苷的存在。
Nucleic Acids Res. 1989 Feb 11;17(3):865-82. doi: 10.1093/nar/17.3.865.
2
Identification and structural characterization of O-beta-ribosyl-(1"----2')-adenosine-5"-phosphate in yeast methionine initiator tRNA.酵母甲硫氨酸起始tRNA中O-β-核糖基-(1"----2')-腺苷-5"-磷酸的鉴定与结构表征
Nucleic Acids Res. 1990 Oct 25;18(20):5989-93. doi: 10.1093/nar/18.20.5989.
3
O-ribosyl-phosphate purine as a constant modified nucleotide located at position 64 in cytoplasmic initiator tRNAs(Met) of yeasts.O-核糖基磷酸嘌呤作为一种恒定的修饰核苷酸，位于酵母细胞质起始tRNA(Met)的第64位。
Nucleic Acids Res. 1991 Oct 11;19(19):5199-203. doi: 10.1093/nar/19.19.5199.
4
Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met).具有酵母起始tRNA(Met)序列的化学合成RNA的特性分析
FEBS Lett. 1990 Aug 20;269(1):60-4. doi: 10.1016/0014-5793(90)81118-8.
5
Discrimination between initiation and elongation of protein biosynthesis in yeast: identity assured by a nucleotide modification in the initiator tRNA.酵母中蛋白质生物合成起始与延伸的区分：由起始tRNA中的核苷酸修饰确保识别。
Nucleic Acids Res. 1993 Dec 11;21(24):5679-83. doi: 10.1093/nar/21.24.5679.
6
Synthesis of RNA containing O-beta-D-ribofuranosyl-(1''-2')-adenosine-5''-phosphate and 1-methyladenosine, minor components of tRNA.含O-β-D-呋喃核糖基-（1''-2'）-腺苷-5''-磷酸和1-甲基腺苷的RNA的合成，tRNA的次要成分
Chem Biodivers. 2005 Sep;2(9):1153-63. doi: 10.1002/cbdv.200590085.
7
Mutational analysis of conserved positions potentially important for initiator tRNA function in Saccharomyces cerevisiae.酿酒酵母中对起始tRNA功能可能重要的保守位点的突变分析。
Mol Cell Biol. 1992 Apr;12(4):1432-42. doi: 10.1128/mcb.12.4.1432-1442.1992.
8
The yeast initiator tRNAMet can act as an elongator tRNA(Met) in vivo.酵母起始tRNAMet在体内可作为延伸tRNA(Met)发挥作用。
J Mol Biol. 1993 Sep 5;233(1):43-58. doi: 10.1006/jmbi.1993.1483.
9
A novel modified nucleoside found at the first position of the anticodon of methionine tRNA from bovine liver mitochondria.在牛肝线粒体甲硫氨酸tRNA反密码子第一位发现的一种新型修饰核苷。
Biochemistry. 1994 Mar 1;33(8):2234-9. doi: 10.1021/bi00174a033.
10
A functional analysis of the repeated methionine initiator tRNA genes (IMT) in yeast.
Mol Gen Genet. 1989 Apr;216(2-3):276-86. doi: 10.1007/BF00334366.
引用本文的文献
1
Adaptation and Exaptation: From Small Molecules to Feathers.适应与特化：从小分子到羽毛。
J Mol Evol. 2022 Apr;90(2):166-175. doi: 10.1007/s00239-022-10049-1. Epub 2022 Mar 4.
2
Naturally occurring modified ribonucleosides.天然存在的修饰核苷。
Wiley Interdiscip Rev RNA. 2020 Sep;11(5):e1595. doi: 10.1002/wrna.1595. Epub 2020 Apr 16.
3
Identification and modeling of a phosphatase-like domain in a tRNA 2'-O-ribosyl phosphate transferase Rit1p.鉴定并构建 tRNA 2′-O-磷酸核糖转移酶 Rit1p 中的磷酸酶样结构域。
Cell Cycle. 2011 Oct 15;10(20):3566-70. doi: 10.4161/cc.10.20.17857.
4
Eukaryotic initiator tRNA: finely tuned and ready for action.真核生物起始tRNA：精确调控且准备就绪发挥作用。
FEBS Lett. 2010 Jan 21;584(2):396-404. doi: 10.1016/j.febslet.2009.11.047.
5
Yeast initiator tRNA identity elements cooperate to influence multiple steps of translation initiation.酵母起始tRNA识别元件协同作用以影响翻译起始的多个步骤。
RNA. 2006 May;12(5):751-64. doi: 10.1261/rna.2263906. Epub 2006 Mar 24.
6
Initiator-elongator discrimination in vertebrate tRNAs for protein synthesis.脊椎动物用于蛋白质合成的tRNA中起始子-延伸子的区分
Mol Cell Biol. 1998 Mar;18(3):1459-66. doi: 10.1128/MCB.18.3.1459.
7
2'-O-methyl-5-formylcytidine (f5Cm), a new modified nucleotide at the 'wobble' of two cytoplasmic tRNAs Leu (NAA) from bovine liver.2'-O-甲基-5-甲酰胞苷（f5Cm），一种存在于来自牛肝的两种细胞质亮氨酰tRNA（NAA）“摆动”位置的新型修饰核苷酸。
Nucleic Acids Res. 1996 Apr 15;24(8):1489-96. doi: 10.1093/nar/24.8.1489.
8
A very poorly expressed tRNA(Ser) is highly concentrated together with replication primer initiator tRNA(Met) in the yeast Ty1 virus-like particles.一种表达水平极低的tRNA(Ser)与复制引物起始tRNA(Met)在酵母Ty1病毒样颗粒中高度聚集在一起。
Nucleic Acids Res. 1993 Apr 11;21(7):1517-21. doi: 10.1093/nar/21.7.1517.
9
Discrimination between initiation and elongation of protein biosynthesis in yeast: identity assured by a nucleotide modification in the initiator tRNA.酵母中蛋白质生物合成起始与延伸的区分：由起始tRNA中的核苷酸修饰确保识别。
Nucleic Acids Res. 1993 Dec 11;21(24):5679-83. doi: 10.1093/nar/21.24.5679.
10
Multiple molecular determinants for retrotransposition in a primer tRNA.引物tRNA中逆转录转座的多个分子决定因素。
Mol Cell Biol. 1995 Jan;15(1):217-26. doi: 10.1128/MCB.15.1.217.
本文引用的文献
1
A GENERAL PROCEDURE FOR THE ISOLATION OF "MINOR" NUCLEOSIDES FROM RIBONUCLEIC ACID HYDROLYSATES.从核糖核酸水解物中分离“次要”核苷的一般方法。
Biochemistry. 1965 Apr;4:661-70. doi: 10.1021/bi00880a008.
2
Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides.tRNA的定量酶促水解：tRNA核苷的反相高效液相色谱法
J Chromatogr. 1982 Jul 9;230(2):297-308.
3
Quantitative high-performance liquid chromatography analysis of modified nucleosides in physiological fluids, tRNA, and DNA.生理体液、转运核糖核酸（tRNA）和脱氧核糖核酸（DNA）中修饰核苷的定量高效液相色谱分析。
Recent Results Cancer Res. 1983;84:344-59. doi: 10.1007/978-3-642-81947-6_26.
4
5
Primary structure of Bombyx mori posterior silkgland tRNAPhe.
Biochem Biophys Res Commun. 1980 Jan 15;92(1):109-15. doi: 10.1016/0006-291x(80)91526-0.
6
Structure of poly(ADP-ribose).
Methods Enzymol. 1984;106:441-50. doi: 10.1016/0076-6879(84)06048-1.
7
Primary structure of three tRNAs from brewer's yeast: tRNAPro2, tRNAHis1 and tRNAHis2.来自酿酒酵母的三种tRNA的一级结构：tRNAPro2、tRNAHis1和tRNAHis2。
Biochimie. 1983 Nov-Dec;65(11-12):661-72. doi: 10.1016/s0300-9084(84)80030-9.
8
Identification of nucleosides in hydrolysates of transfer RNA by high-resolution mass spectrometry.通过高分辨率质谱法鉴定转移核糖核酸水解产物中的核苷
Eur J Biochem. 1982 Oct;127(3):459-71. doi: 10.1111/j.1432-1033.1982.tb06894.x.
9
[Fractionation of Brewer's yeast t-RNA by countercurrent distribution].[通过逆流分配法对啤酒酵母转移核糖核酸进行分级分离]
Bull Soc Chim Biol (Paris). 1967;49(12):1679-87.
10
Nucleotide sequences of rat liver serine-tRNA. 1. Products of digestion with pancreatic ribonuclease.大鼠肝脏丝氨酸 - tRNA的核苷酸序列。1. 胰核糖核酸酶消化产物
Eur J Biochem. 1971 Jul 29;21(2):235-42. doi: 10.1111/j.1432-1033.1971.tb01461.x.
Zotero 插件