Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
FEMS Microbiol Lett. 2011 Feb;315(1):54-62. doi: 10.1111/j.1574-6968.2010.02177.x. Epub 2010 Dec 22.
An oligopeptide permease family ATP-binding cassette (ABC) transporter encoded by SGR2418-SGR2414 was shown to be essential for aerial mycelium formation on glucose-containing media in Streptomyces griseus. In spite of only weak sequence similarity, the operon was equivalent to the bldK operon of Streptomyces coelicolor A3(2) in terms of chromosomal location and function. Transcription of the operon appeared not to be directly regulated by AdpA, a global regulator of morphological and physiological development in S. griseus, although it was affected by adpA inactivation. This study revealed that an ABC transporter was essential for aerial mycelium formation not only in S. coelicolor A3(2) but also in S. griseus, indicating that extracellular signaling by certain peptides should be conserved among different Streptomyces species.
寡肽渗透酶家族 ABC 转运蛋白由 SGR2418-SGR2414 编码，被证明在葡萄糖存在的培养基中对灰色链霉菌气生菌丝体的形成是必需的。尽管序列相似性很弱，但该操纵子在染色体位置和功能方面与链霉菌 A3(2)的 bldK 操纵子相当。尽管该操纵子的转录受到 adpA 失活的影响，但似乎不受全局调节因子 AdpA 的直接调控，AdpA 是灰色链霉菌形态和生理发育的全局调节因子。这项研究表明，ABC 转运蛋白不仅在链霉菌 A3(2)中，而且在灰色链霉菌中，对气生菌丝体的形成都是必需的，这表明某些肽的细胞外信号应该在不同的链霉菌物种中保守。
