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人转铁蛋白的N-连接寡糖对于与细菌转铁蛋白受体的结合并非必需。

N-linked oligosaccharides of human transferrin are not required for binding to bacterial transferrin receptors.

作者信息

Padda J S, Schryvers A B

机构信息

Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.

出版信息

Infect Immun. 1990 Sep;58(9):2972-6. doi: 10.1128/iai.58.9.2972-2976.1990.

DOI:10.1128/iai.58.9.2972-2976.1990
PMID:2117577
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313597/
Abstract

Derivatives of human transferrin (hTf) with removed or modified N-linked oligosaccharides were compared with native hTf with respect to their binding to bacterial hTf receptors from Neisseria meningitidis, N. gonorrhoeae, and Haemophilus influenzae. Partially and fully deglycosylated hTf were prepared by enzymatic deglycosylation with glycopeptidase F and isolated by concanavalin A-Sepharose affinity chromatography. Oligosaccharide-modified hTf was prepared via mild periodate oxidation. Competition and direct binding experiments with the hTf derivatives demonstrated that the hTf oligosaccharides are not essential for binding to the bacterial hTf receptors.

摘要

将去除或修饰了N - 连接寡糖的人转铁蛋白（hTf）衍生物，与天然hTf在与脑膜炎奈瑟菌、淋病奈瑟菌和流感嗜血杆菌的细菌hTf受体结合方面进行了比较。通过糖肽酶F进行酶促去糖基化制备部分和完全去糖基化的hTf，并通过伴刀豆球蛋白A - 琼脂糖亲和色谱法进行分离。通过温和的高碘酸盐氧化制备寡糖修饰的hTf。用hTf衍生物进行的竞争和直接结合实验表明，hTf寡糖对于与细菌hTf受体的结合不是必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/3858eaf84186/iai00057-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/8842aab31cc9/iai00057-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/f47f46a84111/iai00057-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/24cbbcca5534/iai00057-0261-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/3858eaf84186/iai00057-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/8842aab31cc9/iai00057-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/f47f46a84111/iai00057-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/24cbbcca5534/iai00057-0261-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b99/313597/3858eaf84186/iai00057-0262-a.jpg

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