Uzan G, Frain M, Park I, Besmond C, Maessen G, Trépat J S, Zakin M M, Kahn A
Biochem Biophys Res Commun. 1984 Feb 29;119(1):273-81. doi: 10.1016/0006-291x(84)91648-6.
A cDNA clone for human transferrin was identified from a human liver cDNA library by pre-screening with different ss-cDNA probes against length-fractionated liver mRNAs, positive hybridization-selection and nucleotide sequence analysis. The insert was of 1 kb, encoding human transferrin from aminoacid 403 through the COOH terminus, with a 3' non coding region of 166 nucleotides. This insert hybridized with a single major mRNA species of about 2.4 kb and several genomic DNA restriction fragments. Hybridization of the Southern blots with different parts of the transferrin insert and at different stringences suggest that the various bands observed correspond to splice sites inside one gene rather than to hybridization to several related genes. Finally, a single or a low number of transferrin gene copies seem to exist in the human genome.
通过使用针对长度分级的肝脏mRNA的不同单链cDNA探针进行预筛选、阳性杂交选择和核苷酸序列分析，从人肝脏cDNA文库中鉴定出了人转铁蛋白的一个cDNA克隆。插入片段为1 kb，从氨基酸403到COOH末端编码人转铁蛋白，具有166个核苷酸的3'非编码区。该插入片段与一个约2.4 kb的主要mRNA种类以及几个基因组DNA限制片段杂交。用转铁蛋白插入片段的不同部分在不同严谨度下进行Southern印迹杂交表明，观察到的各种条带对应于一个基因内的剪接位点，而不是与几个相关基因的杂交。最后，人基因组中似乎存在单个或少量的转铁蛋白基因拷贝。
