Department of Neuropsychiatry, Hallym University, Hangang Sacred Heart Hospital, Seoul, Republic of Korea.
Genes Brain Behav. 2011 Mar;10(2):253-6. doi: 10.1111/j.1601-183X.2010.00663.x. Epub 2010 Dec 28.
The cholinergic muscarinic 2 receptor (CHRM2) gene has been considered a candidate gene for the alcohol dependence in that it might underpin certain risk factors for this condition. This study examined variations in the CHRM2 between the patients with alcohol dependence and population controls in Korean and explored the associations between CHRM2 polymorphisms and severity of symptoms in the patients with alcohol dependence. One hundred and fifty-five patients with alcohol dependence, defined by the Alcohol Use Disorders Identification Test (AUDIT) and the Alcohol Dependence Scale (ADS) to measure the severity of symptoms, and one hundred and ninety-five population controls were drawn in the study. Three single nucleotide polymorphisms (SNPs) of CHRM2 were genotyped using the TaqMan assay and analyzed with the severity of symptoms of alcohol dependence. We found that although SNP rs324650 showed marginal association with the risk of alcohol dependence (P = 0.03), the significance of the result was not sustained after multiple corrections. SNP rs1824024 was significantly associated with the AUDIT and ADS scores in patients (P = 0.005 and 0.003, respectively). These findings suggested that the muscarinic acetylcholine function might be related not with alcohol dependence itself but with the severity of alcohol dependence in Korean population.
乙酰胆碱能毒蕈碱 2 型受体（CHRM2）基因被认为是酒精依赖的候选基因，因为它可能是这种疾病某些风险因素的基础。本研究在韩国的酒精依赖患者和普通人群中检测了 CHRM2 的变异，并探讨了 CHRM2 多态性与酒精依赖患者症状严重程度之间的关系。该研究纳入了 155 名酒精依赖患者（根据酒精使用障碍识别测试（AUDIT）和酒精依赖量表（ADS）来衡量症状严重程度）和 195 名普通人群对照。使用 TaqMan 检测法对 CHRM2 的三个单核苷酸多态性（SNP）进行基因分型，并分析其与酒精依赖症状严重程度的关系。我们发现，尽管 SNP rs324650 与酒精依赖的风险呈边缘关联（P = 0.03），但在多次校正后，该结果的意义并不持续。SNP rs1824024 与患者的 AUDIT 和 ADS 评分显著相关（分别为 P = 0.005 和 0.003）。这些发现表明，在韩国人群中，毒蕈碱乙酰胆碱功能可能与酒精依赖本身无关，而是与酒精依赖的严重程度有关。
