Gong Hong-li, Shi Yi, Zhou Liang, Tao Lei, Ji Jian, Chen Hui-wen
Department of Otorhinolarnygology, Fudan University, Shanghai 200031, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Oct;45(10):839-42.
To investigate the difference in Helicobacter pylori (Hp) infection rate between the patients with laryngeal squamous cell carcinoma and the patients with benign laryngeal lesions and to explore the role of Hp infection in the pathogenesis of laryngeal squamous carcinoma.
Nested polymerase chain reaction (nPCR) and Hp culture were used to identify the Hp in laryngeal mucosa in 30 patients with laryngeal carcinoma and 15 patients with benign laryngeal lesions including polyps of vocal cords or epiglottic cysts.
nPCR showed that Hp-positive rate (73.3%) of patients with laryngeal carcinoma was significantly higher than that (20.0%) of control patients with benign laryngeal lesions (χ(2) = 11.520, P = 0.010). Regarding the 22 positive neoplastic cases that have 44 tissues, out of the 22 tumor tissues, 10 were positive with Hp (45.4%), and among 22 tissues beside the tumor, 19 were positive (86.4%). This event also indicated a statistical significance (χ(2) = 4.697, P = 0.030). Hp culture showed that Hp was negative in all specimens.
Hp exist in the laryngeal mucosa, but with the higher rate of Hp infection in the patients with laryngeal squamous cell carcinoma than that in the patients with benign laryngeal lesions, which suggests Hp may be one of pathogenic factors of laryngeal squamous cell carcinoma.
探讨喉鳞状细胞癌患者与喉良性病变患者幽门螺杆菌（Hp）感染率的差异，以及Hp感染在喉鳞状细胞癌发病机制中的作用。
采用巢式聚合酶链反应（nPCR）和Hp培养法，对30例喉癌患者及15例喉良性病变患者（包括声带息肉或会厌囊肿）的喉黏膜进行Hp检测。
nPCR检测显示，喉癌患者的Hp阳性率（73.3%）显著高于喉良性病变对照组患者（20.0%）（χ² = 11.520，P = 0.010）。在22例呈阳性的肿瘤病例共44份组织中，22份肿瘤组织中10份Hp阳性（45.4%），肿瘤旁22份组织中19份阳性（86.4%）。此结果也具有统计学意义（χ² = 4.697，P = 0.030）。Hp培养显示所有标本均为Hp阴性。
Hp存在于喉黏膜中，且喉鳞状细胞癌患者的Hp感染率高于喉良性病变患者，提示Hp可能是喉鳞状细胞癌的致病因素之一。
