Logan T M, Linthicum K J, Bailey C L, Watts D M, Dohm D J, Moulton J R
United States Army Medical Research Unit-Kenya, APO NY 09675.
J Med Entomol. 1990 Jul;27(4):537-42. doi: 10.1093/jmedent/27.4.537.
The vector potentials of Hyalomma dromedarii Koch, H. impeltatum Schulze & Schlottke, H. truncatum Koch, and Rhipicephalus appendiculatus Neumann for Crimean-Congo hemorrhagic fever (CCHF) virus (IbAr 10200) were evaluated by intracoelomic inoculation. All three Hyalomma species became infected; infection rates ranged between 80 and 100% at 7-14 d after inoculation, and viral titers increased in unfed specimens almost 100 times above inoculation levels within the first week following infection. Only 40% of the R. appendiculatus became infected, and viral titers of unfed specimens increased less than 10 times above inoculation levels. The virus persisted to 153 d in unfed H. impeltatum. Viral titers were significantly higher in female than in male H. dromedarii, H. impeltatum, H. truncatum, and R. appendiculatus after blood feeding. Blood feeding had little effect on the viral titers of male Hyalomma species. However, the percentage of female and male ticks from which virus was recovered was significantly higher from fed ticks compared with unfed ticks. No virological evidence of transovarial transmission was found in greater than 78,000 first-generation progeny (larvae, nymphs, and adults) of inoculated female H. dromedarii, H. impeltatum, H. truncatum, and R. appendiculatus. All species transmitted CCHF virus to guinea pigs when allowed to feed at both 6 and 21 d after inoculation.
通过体腔接种评估了单峰驼璃眼蜱、施氏璃眼蜱、截形璃眼蜱和肩突硬蜱传播克里米亚-刚果出血热（CCHF）病毒（IbAr 10200）的可能性。三种璃眼蜱均被感染；接种后7-14天感染率在80%至100%之间，未进食标本的病毒滴度在感染后的第一周内增加到接种水平的近100倍。只有40%的肩突硬蜱被感染，未进食标本的病毒滴度增加不到接种水平的10倍。病毒在未进食的施氏璃眼蜱中持续存在至153天。吸血后，雌性单峰驼璃眼蜱、施氏璃眼蜱、截形璃眼蜱和肩突硬蜱的病毒滴度显著高于雄性。吸血对雄性璃眼蜱的病毒滴度影响不大。然而，与未进食的蜱相比，从进食的蜱中回收病毒的雌性和雄性蜱的百分比显著更高。在接种的雌性单峰驼璃眼蜱、施氏璃眼蜱、截形璃眼蜱和肩突硬蜱的超过78,000只第一代后代（幼虫、若虫和成虫）中未发现经卵传播的病毒学证据。所有物种在接种后6天和21天进食时均将CCHF病毒传播给豚鼠。
