Gordon S W, Linthicum K J, Moulton J R
Department of Epidemiology, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland.
Am J Trop Med Hyg. 1993 Apr;48(4):576-80. doi: 10.4269/ajtmh.1993.48.576.
Crimean-Congo hemorrhagic fever (CCHF) virus was transmitted from infected adult Hyalomma ticks to uninfected larval and nymphal Hyalomma ticks while cofeeding on a guinea pig host that did not have a detectable viremia. When tested after feeding with infected adults, three (0.8%) of 370 H. truncatum larvae contained detectable CCHF virus (mean virus titer 10(1.6) plaque-forming units [PFU]/tick). The virus was transmitted transstadially from infected larvae and was detected in 15 (1.2%) of 1,253 nymphs and 12 (0.1%) of 2,049 adults. Virus was recovered from 18 (1.9%) of 931 H. impeltatum nymphs, which originated from larvae that cofed with infected adults. After H. impeltatum nymphs cofed with infected adults, CCHF virus was detected in 21 (4.3%) of 449 (mean virus titer 10(1.7) PFU/tick) fed nymphs, but none of 886 adults tested after molt. Results of this study indicate that a small proportion of either larvae or nymphs may acquire CCHF infection while cofeeding on a host without a detectable viremia.
克里米亚-刚果出血热（CCHF）病毒在未感染的璃眼蜱幼虫和若虫与感染的成年璃眼蜱在一只无检测到病毒血症的豚鼠宿主身上共同取食时，从感染的成年璃眼蜱传播到未感染的幼虫和若虫身上。在用感染的成年蜱喂食后进行检测时，370只截形璃眼蜱幼虫中有3只（0.8%）含有可检测到的CCHF病毒（平均病毒滴度为10(1.6) 蚀斑形成单位 [PFU]/蜱）。病毒经变态从感染的幼虫传播，在1253只若虫中有15只（1.2%）和2049只成虫中有12只（0.1%）检测到该病毒。从931只起源于与感染成年蜱共同取食的幼虫的刻点璃眼蜱若虫中的18只（1.9%）中分离到病毒。刻点璃眼蜱若虫与感染成年蜱共同取食后，在449只取食后的若虫中有21只（4.3%）检测到CCHF病毒（平均病毒滴度为10(1.7) PFU/蜱），但蜕皮后的886只成虫检测均为阴性。本研究结果表明，一小部分幼虫或若虫在无检测到病毒血症的宿主身上共同取食时可能获得CCHF感染。
