Department of Biology, São Paulo State University, Rio Claro, São Paulo, Brazil.
Tissue Cell. 2011 Feb;43(1):24-8. doi: 10.1016/j.tice.2010.11.001. Epub 2010 Dec 19.
Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35 mg/kg). Training program consisted of swimming 1h/day with a load of 4.8% of body weight for TD and 5.2% for TC, during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic acid of Schiff, picrosirius-hematoxylin and ammoniacal silver. The PAS technique shows that individuals of group SD presented more intense reaction that the other groups. Picrosirius-hematoxylin technique showed a possible deposition of collagen fibers in SD. The TD group presented a reaction a lot similar to the controls' for both techniques', showing a possible prevention of this deposition. These results indicate that physical exercises might have an important role on the prevention of some negative alterations caused by experimental diabetes.
糖尿病性心肌病导致两种类型糖尿病的死亡率都很高。我们旨在研究心肌胶原和网状纤维的组织化学方面，并评估体育锻炼对这些方面的影响。Wistar 大鼠分为 4 组：久坐对照组（SC）、训练对照组（TC）、久坐糖尿病组（SD）和训练糖尿病组（TD）。糖尿病通过链脲佐菌素（35mg/kg）诱导。训练计划包括每天游泳 1 小时，TD 组的负荷为体重的 4.8%，TC 组为 5.2%，持续 8 周。实验结束时，采集左心室样本，用 Bouin 液固定，用组织树脂处理。切片用过碘酸-Schiff、苦味酸-hematoxylin 和氨银染色。PAS 技术显示 SD 组的反应比其他组更强烈。苦味酸-hematoxylin 技术显示 SD 组可能有胶原纤维沉积。TD 组对两种技术的反应与对照组非常相似，表明可能预防了这种沉积。这些结果表明，体育锻炼可能对预防实验性糖尿病引起的一些负面改变有重要作用。
