多发性硬化症（MS）CSF IgG 与 MS CSF 中克隆扩增的浆细胞产生的重组抗体之间的肽反应性。
Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF.
机构信息
Department of Neurology, University of Colorado Denver at Anschutz Medical Campus, 12700 E. 19th Ave., Aurora, CO 80045, United States.
出版信息
J Neuroimmunol. 2011 Apr;233(1-2):192-203. doi: 10.1016/j.jneuroim.2010.11.007. Epub 2010 Dec 21.
Abstract
We employed 19 recombinant antibodies (rAbs) generated from clonally expanded plasma cells, and native IgG from cerebrospinal fluid (CSF) of three multiple sclerosis (MS) patients for panning with phage displayed random peptide libraries. Specific peptide epitopes/mimotopes were identified and characterized. Importantly, peptide-antibody interactions were shared by rAbs and native IgG from the same patient. Three peptides strongly interacted with at least one other MS CSF, but not to inflammatory CNS controls. Database searches revealed several protein candidates including stress proteins, cell surface proteins, and neuronal proteins. Peptides derived from the candidate proteins were recognized by rAbs. Identification of peptide epitopes/mimotopes in MS may provide clues regarding disease-relevant antigens.
摘要
我们使用了 19 种从克隆扩增的浆细胞产生的重组抗体（rAb）和 3 名多发性硬化症（MS）患者的脑脊液（CSF）中的天然 IgG，用于用噬菌体展示的随机肽文库进行淘选。鉴定和表征了特定的肽表位/模拟表位。重要的是，来自同一患者的 rAb 和天然 IgG 共享肽-抗体相互作用。三种肽与至少一种其他 MS CSF 强烈相互作用，但不与炎症性中枢神经系统对照相互作用。数据库搜索显示了包括应激蛋白、细胞表面蛋白和神经元蛋白在内的几种候选蛋白。候选蛋白衍生的肽被 rAb 识别。鉴定 MS 中的肽表位/模拟表位可能为疾病相关抗原提供线索。
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