Winges Kimberly M, Gilden Donald H, Bennett Jeffrey L, Yu Xiaoli, Ritchie Alanna M, Owens Gregory P
Howard Hughes Medical Institute Research Fellow, Chevy Chase, MD 20815, USA.
J Neuroimmunol. 2007 Dec;192(1-2):226-34. doi: 10.1016/j.jneuroim.2007.10.009. Epub 2007 Nov 13.
Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89+/-2% (mean+/-SE) of the CD138+ cell population (P<0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11+/-2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.
对15例临床确诊的多发性硬化症患者的17份脑脊液样本中的B细胞亚型进行荧光激活细胞分选（FACS）分析，结果显示CD19⁺ B细胞占脑脊液淋巴细胞总数的2%至11%（平均5%），CD138⁺细胞占0%至5%（平均2%）。基于CD19表达水平对CD138⁺细胞进行进一步分层分析显示，CD138⁺19⁺浆母细胞占CD138⁺细胞群体的89±2%（平均值±标准误）（P<0.00001），其余11±2%为更成熟的浆细胞（CD138⁺19⁻）。对从多发性硬化症脑脊液中分选的单个CD138⁺19⁺和CD138⁺19⁻细胞的免疫球蛋白可变区进行序列分析，发现在这两个群体中鉴定出许多相同的克隆群体，表明存在一系列克隆相关的浆细胞亚型，其中CD138⁺19⁺浆母细胞最为丰富。
