He Mu-dan, Xu Tian-xing, Wang Xiao-ping, Li Ya-lan, Liu Yu-yong, Duan Shan-e
Department of Anesthesiology, First Affiliated Hospital of Jinan University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Dec;30(12):2637-9.
To detect the self-assembly morphology of sodium hyaluronate injection on mica using atomic force microscopy(AFM).
Atomic force microscopy with nanometer resolution was used to observe the self-assembly morphology of different concentrations of sodium hyaluronate injection on mica at room temperature.
The self-assembly morphology of 0.001, 0.01, and 0.1 mg/ml sodium hyaluronate injection on mica featured piebald, reticular and dendritic structures, respectively. At 1 and 5 mg/ml, sodium hyaluronate injection displayed bacilliform and spherical structures on mica, respectively; the diameter and height of the particles of 5 mg/ml sodium hyaluronate was 197.97±78.48 nm and 30.79±18.67 nm, significantly greater than those of 0.1 mg/ml sodium hyaluronate injection (49.52±11.93 nm and 5.37±1.59 nm, respectively, P<0.05).
The self-assembly morphology of sodium hyaluronate injection on mica varies with its concentration. The piebald and reticular structure may facilitate the function of sodium hyaluronate, and the dendritic feature resembles the representative model of diffusion-limited aggregation (DLA).
采用原子力显微镜（AFM）检测透明质酸钠注射液在云母片上的自组装形态。
运用具有纳米分辨率的原子力显微镜，在室温下观察不同浓度透明质酸钠注射液在云母片上的自组装形态。
0.001、0.01和0.1mg/ml透明质酸钠注射液在云母片上的自组装形态分别呈现为花斑状、网状和树枝状结构。在1和5mg/ml时，透明质酸钠注射液在云母片上分别呈现杆状和球状结构；5mg/ml透明质酸钠颗粒的直径和高度分别为197.97±78.48nm和30.79±18.67nm，显著大于0.1mg/ml透明质酸钠注射液的（分别为49.52±11.93nm和5.37±1.59nm，P<0.05）。
透明质酸钠注射液在云母片上的自组装形态随其浓度而变化。花斑状和网状结构可能有助于透明质酸钠发挥功能，而树枝状特征类似于扩散限制聚集（DLA）的典型模型。
