Zhang Peng, DU Yu-bin, Yu Miao, Yin Xiang, Lv Yan-hong, Gao Zhong-xiu-zi, Zheng Jin-hua
Department of Anatomy, Harbin Medical University, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Dec;30(12):2696-8.
To investigate the relationship between the extracellular matrix (ECM) and neoplastic progression in hamster with tongue cancer.
Forty-eight specimens of hamster tongue cancer were divided into control group (n=6) and experimental group (n=42). The pathological grade of the specimens was assessed (including 3 stages, namely atypical hyperplasia, carcinoma in situ and early invasive carcinoma). The sections of the tongue were stained with Masson and aldehyde-fuchsin (AF) staining for microscopic observation of the elastic fiber and collagen fiber changes.
Within the connective tissue cores (CTC) of the papillae in the control group was a framework of numerous and fine Gomrori's aldehyde fuchsin-positive elastic fibers. But in the stages of dysplasia and carcinoma in situ, these elastic fibers decreased and further diminished in the CTC in early invasive carcinoma. In dysplasia and carcinoma in situ stages, most of the elastic fibers collapsed with scattered elastic fibers, and the elastic fibers decreased significantly in early invasive carcinoma. The control group showed a significantly greater number of elastic fibers in the experimental group. The collagen fiber was obviously increased and irregularly arranged in dysplasia and carcinoma in situ stage; in early invasive carcinoma, the collagen fibers became thicker with deposition in the lamina propria.
An excessive deposition of collagen fiber and reduction of the elastic fibers is an important factor contributing to the development of tongue carcinoma in hamsters.
探讨仓鼠舌癌中细胞外基质（ECM）与肿瘤进展之间的关系。
将48例仓鼠舌癌标本分为对照组（n = 6）和实验组（n = 42）。对标本进行病理分级评估（包括3个阶段，即非典型增生、原位癌和早期浸润癌）。对舌组织切片进行Masson染色和醛复红（AF）染色，显微镜下观察弹性纤维和胶原纤维的变化。
对照组乳头结缔组织核心（CTC）内有许多细小的Gomori醛复红阳性弹性纤维构成的框架。但在发育异常和原位癌阶段，这些弹性纤维减少，在早期浸润癌的CTC中进一步减少。在发育异常和原位癌阶段，大多数弹性纤维塌陷，弹性纤维散在分布，在早期浸润癌中弹性纤维显著减少。对照组的弹性纤维数量明显多于实验组。胶原纤维在发育异常和原位癌阶段明显增多且排列不规则；在早期浸润癌中，胶原纤维变粗并沉积于固有层。
胶原纤维过度沉积和弹性纤维减少是仓鼠舌癌发生发展的重要因素。
