Urogenital Research Unit, State University of Rio de Janerio, Rio de Janerio, RJ, Brazil.
BJU Int. 2011 Aug;108(3):400-4. doi: 10.1111/j.1464-410X.2010.09944.x. Epub 2010 Dec 16.
OBJECTIVE • To assess the volumetric density of collagen, elastic system fibres and smooth muscle cells in the corpus cavernosum (CC), corpus spongiosum (CS) and tunica albuginea (TA) in the penis of diabetic rabbits.
• Twenty-six New Zealand white rabbits were used. Diabetes was induced at 8 weeks of age in 13 rabbits by i.v. injection of 100 mg/kg of alloxan. The remaining 13 rabbits served as a control group. After 10 weeks, the rabbits were killed using sodium thiopenthal. • Midshaft penile fragments were obtained and processed by routine histological techniques. Stereological analysis of collagen, elastic system fibres and smooth muscle was performed in 5-µm sections by using a M42 test grid system. • Data were expressed as volumetric density (Vv; %). Collagen organization was evaluated by Picrosirius red staining under polarization.
• In the TA of diabetic rabbits, thickness increased by 88% (P < 0.001) with an enhanced collagen turnover. Moreover, the elastic fibre content was 34% higher (P < 0.001). In the CC of diabetics, collagen was diminished by 45% (P < 0.001) with a more organized collagen. • The elastic fibres were decreased by 46% (P < 0.001). Diabetes induced a 11% increase in CS collagen (P < 0.024) with an enhanced collagen turnover. • Smooth muscle in the CC of diabetic rabbits was increased by 40% (P < 0.001), whereas, in the CS, it was decreased by a similar amount (P < 0.001).
• Penile tissues were affected differently by diabetes, possibly as a result of cellular heterogeneity. • These changes could have an impact on blood flow and tissue resistance, and therefore might adversely affect erection.
评估糖尿病兔阴茎海绵体（CC）、海绵体（CS）和白膜（TA）中胶原、弹性纤维系统和平滑肌细胞的体积密度。
共使用 26 只新西兰白兔。13 只兔子在 8 周龄时经静脉注射 100mg/kg 链脲佐菌素诱导糖尿病。其余 13 只兔子作为对照组。10 周后，用硫喷妥钠处死兔子。
从中段阴茎段获得组织标本，并用常规组织学技术处理。用 M42 测试网格系统在 5µm 切片上对胶原、弹性纤维系统和平滑肌进行体视学分析。
数据以体积密度（Vv；%）表示。用偏振光下的苦味酸天狼猩红染色评估胶原组织。
糖尿病兔 TA 厚度增加 88%（P<0.001），胶原周转率增加。此外，弹性纤维含量增加 34%（P<0.001）。糖尿病兔 CC 中胶原减少 45%（P<0.001），胶原更有组织。
弹性纤维减少 46%（P<0.001）。CS 胶原增加 11%（P<0.024），糖尿病诱导胶原周转率增加。
糖尿病兔 CC 平滑肌增加 40%（P<0.001），而 CS 平滑肌减少相同数量（P<0.001）。
阴茎组织受糖尿病影响不同，可能是由于细胞异质性。这些变化可能对血流和组织阻力产生影响，从而可能对勃起产生不利影响。
