Haluska G J, Mitchell M D, Novy M J
Division of Reproductive Biology & Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
Prostaglandins. 1990 Jul;40(1):99-105. doi: 10.1016/0090-6980(90)90060-9.
To determine changes in amniotic fluid (AF) lipoxygenase metabolites prior to spontaneous labor and after RU486 administration, we implanted AF and vascular catheters and myometrial electromyographic (EMG) electrodes in 8 rhesus macaques at 120-130 days of pregnancy (term = 167 days). Four animals had AF samples taken serially until they delivered their infants normally at term. The other four animals received RU486 (20 mg/kg/day) for 3 days. AF samples were collected every 2-3 days and at 12 hour intervals for 72 hours before and after treatment with RU486. Uterine activity was monitored continuously. LTB4, 5-HETE and 15-HETE were measured by radioimmunoassay. In untreated animals, LTB4 and 5-HETE concentrations in AF increased significantly (P less than 0.05) 4 days before delivery with no change in 15-HETE. After RU486, mean levels of LTB4 and 5-HETE were increased although the difference was not statistically significant. No change in 15-HETE levels was observed. In conclusion, LTB4 and 5-HETE increase in AF before the onset of spontaneous labor. Progesterone receptor blockade by RU486 does not reproduce the changes in AF lipoxygenase metabolites observed during normal parturition.
为了确定自然分娩前及给予米非司酮后羊水（AF）中脂氧合酶代谢产物的变化，我们在妊娠120 - 130天（足月为167天）的8只恒河猴身上植入了羊水和血管导管以及子宫肌层肌电图（EMG）电极。4只动物连续采集羊水样本，直至足月正常分娩。另外4只动物接受米非司酮（20 mg/kg/天）治疗3天。在米非司酮治疗前后，每2 - 3天并每隔12小时采集一次羊水样本，共采集72小时。持续监测子宫活动。通过放射免疫分析法测定白三烯B4（LTB4）、5 - 羟基二十碳四烯酸（5 - HETE）和15 - 羟基二十碳四烯酸（15 - HETE）。在未治疗的动物中，分娩前4天羊水中LTB4和5 - HETE浓度显著增加（P小于0.05），而15 - HETE无变化。给予米非司酮后，LTB4和5 - HETE的平均水平有所升高，尽管差异无统计学意义。未观察到15 - HETE水平的变化。总之，自然分娩开始前羊水中LTB4和5 - HETE增加。米非司酮对孕激素受体的阻断并未重现正常分娩期间观察到的羊水脂氧合酶代谢产物的变化。
