Yang Liu, Xu Xiao-fang, Yang Bai-xia, Liu Wen, Li Song
Dept. of Endodontics, College of Stomatology, Anhui Medical University, Hefei 230032, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Oct;28(5):565-9.
To purify mutacin produced from isolated Streptococcus mutans (S. mutans) strains in order to, contribute to molecular biological research of mutacin.
The antibacterial activity of 80 isolated strains was tested by the stab culture technique against Streptococcus oralis ATCC 10557. The mutacin produced by strain 1G, was initially purified by solid-phase extraction (SPE) after crude extraction by chloroform. And then the active substances were purified by twice reversed-phase high performance liquid chromatography (RP-HPLC). The purified target peptide (mutacin) was collected and freeze-dried for further study.
The greatest active strain of these S. mutans isolates, the strain 1G was obtained. Roughly 15 microg crude mutacin was extracted from 200 mL liquid medium of this strain 1G. The purified mutacin through SPE and twice RP-HPLC was obtained.
It was much complex to separate and purify mutacin due to its small molecular mass, and extracting and purifying of mutacin may make an important contribution to the further research of mutacin.
纯化从分离出的变形链球菌菌株产生的变链菌素，以有助于变链菌素的分子生物学研究。
采用针刺培养技术，对80株分离菌株针对口腔链球菌ATCC 10557的抗菌活性进行检测。1G菌株产生的变链菌素，在经氯仿粗提取后，首先通过固相萃取（SPE）进行纯化。然后通过两次反相高效液相色谱（RP-HPLC）对活性物质进行纯化。收集纯化后的目标肽（变链菌素）并冻干以备进一步研究。
获得了这些变形链球菌分离株中活性最强的菌株1G。从该1G菌株的200 mL液体培养基中粗提取得到约15微克变链菌素。通过SPE和两次RP-HPLC获得了纯化的变链菌素。
由于变链菌素分子量小，其分离纯化过程较为复杂，变链菌素的提取和纯化可能对变链菌素的进一步研究有重要贡献。
