Chen Jia-Xu, Yang Jian-Xin, Zhao Xin, Wang Zhu-Feng, Yue Guang-Xin
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Nov;21(4):402-6.
To investigate the changes of glucocorticoid receptor (GR) in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.
We copied the rat model of chronic immobilization stress (180 min daily, repeated 7 days or 21 days), and characterized the changes of GR in hippocampus CA1, cortex, dentate gyrus via immunohistochemistry integrated image analysis.
The contents of glucocorticoid receptors in hippocampus CA1 , cortex, dentate gyrus were increased and the intensity of immunity reaction was significantly stronger in the model group of 7 days than that in the normal control group (P < 0.05). The contents of GR were significantly decreased and the intensity of immunity reaction was weaken in the model group of 21 days (P < 0.05).
Compared with the model group of 21 days, the contents of GR were significantly increased and immunity reaction was intensified in three groups of treatment with Chinese herbs (P < 0.05), in which the group treated with soothing-liver herbs Xiaoyaopowder was the best.
探讨慢性束缚应激大鼠脑区糖皮质激素受体（GR）的变化及中药的影响。
复制大鼠慢性束缚应激模型（每日180分钟，重复7天或21天），采用免疫组化图像分析技术观察海马CA1区、皮质、齿状回GR的变化。
7天模型组海马CA1区、皮质、齿状回糖皮质激素受体含量增加，免疫反应强度明显高于正常对照组（P<0.05）；21天模型组GR含量明显降低，免疫反应强度减弱（P<0.05）。
与21天模型组比较，中药三组均能使GR含量明显升高，免疫反应增强（P<0.05），其中以疏肝中药逍遥散组效果最佳。
