Chen Jia-xu, Li Wei, Zhao Xin, Yang Jian-xin, Xu Hong-yan
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 May;21(2):121-5.
To investigate the changes of enkephalin mRNA and prodynorphin mRNA gene expression in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.
We copied the rat model of chronic immobilization stress (3 h daily , repeated 7 d or 21 d), and primers of enkephalin or prodynorphin were respectively added for RT-PCR reaction (BETA-actin as inner contrast). Gel image analysis system was used to scan and analyze and odds of optical density of target gene and inner contrast strip were taken as quasi-quantified data.
Prodynorphin mRNA expression in hippocampus markedly increased in 7 d group (P < 0.01), while enkephalin mRNA prodynorphin mRNA expression in hippocampus markedly increased in 21 d group (P < 0.01). All three recipes were able to decrease the gene expression of prodynorphin mRNA (P < 0.01), and xiaoyao powder as well as sijunzi soup were able to decrease the gene expression of enkephalin mRNA (P < 0.01).
The effects of xiaoyao powder on the above two gene expression was better than jinkuishenqi pill.
