酵母中调控严格的铜窗。
The tightly regulated copper window in yeast.
机构信息
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.
出版信息
Chem Commun (Camb). 2011 Mar 7;47(9):2571-3. doi: 10.1039/c0cc04292g. Epub 2010 Dec 21.
PMID:21180717
Abstract
The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu(+). The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.
摘要
两种对立的酵母铜调控因子 Ace1 和 Mac1 被转化为两种荧光探针 Ace1-FRET 和 Mac1-FRET，它们可选择性且灵敏地响应 Cu(+)。两种调节剂以及铜储存蛋白 Cup1 和 Crs5 的 Cu(+)结合曲线表明游离铜(i)在酵母内部的一个狭窄窗口内保持。
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