Department of Chemistry and Chemical Engineering, Yulin University, Yulin, 719000, Shaanxi, China.
Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
Sci Rep. 2023 Jul 4;13(1):10761. doi: 10.1038/s41598-023-37838-y.
The extensive usage and production of copper may lead to toxic effects in organisms due to its accumulation in the environment. Traditional methods for copper detection are time consuming and infeasible for field usage. It is necessary to discover a real-time, rapid and economical method for detecting copper to ensure human health and environmental safety. Here we developed a colorimetric paper strip method and optimized spectrum method for rapid detection of copper ion based on the specific copper chelator bathocuproinedisulfonic acid disodium salt (BCS). Both biological assays and chemical methods verified the specificity of BCS for copper. The optimized reaction conditions were 50 mM Tris-HCl pH 7.4, 200 µM BCS, 1 mM ascorbate and less than 50 µM copper. The detection limit of the copper paper strip test was 0.5 mg/L by direct visual observation and the detection time was less than 1 min. The detection results of grape, peach, apple, spinach and cabbage by the optimized spectrum method were 0.91 μg/g, 0.87 μg/g, 0.19 μg/g, 1.37 μg/g and 0.39 μg/g, respectively. The paper strip assays showed that the copper contents of grape, peach, apple, spinach and cabbage were 0.8 mg/L, 0.9 mg/L, 0.2 mg/L, 1.3 mg/L and 0.5 mg/L, respectively. These results correlated well with those determined by inductively coupled plasma-mass spectrometry (ICP-MS). The visual detection limit of the paper strip based on Cu-BCS-AgNPs was 0.06 mg/L. Our study demonstrates the potential for on-site, rapid and cost-effective copper monitoring of foods and the environment.
由于铜在环境中的积累，其广泛的使用和生产可能导致生物体产生毒性作用。传统的铜检测方法耗时且不适用于现场使用。因此，有必要开发一种实时、快速且经济的铜检测方法，以确保人类健康和环境安全。在这里，我们基于特定的铜螯合剂二磺酸钠五水合铜试剂（BCS）开发了一种用于快速检测铜离子的比色纸条法和优化光谱法。生物测定和化学方法都验证了 BCS 对铜的特异性。优化的反应条件为 50 mM Tris-HCl pH 7.4、200 μM BCS、1 mM 抗坏血酸和小于 50 μM 的铜。通过直接目视观察，铜纸条测试的检测限为 0.5 mg/L，检测时间小于 1 分钟。优化光谱法检测葡萄、桃、苹果、菠菜和白菜的结果分别为 0.91μg/g、0.87μg/g、0.19μg/g、1.37μg/g 和 0.39μg/g。纸条检测表明，葡萄、桃、苹果、菠菜和白菜中的铜含量分别为 0.8mg/L、0.9mg/L、0.2mg/L、1.3mg/L 和 0.5mg/L。这些结果与电感耦合等离子体质谱法（ICP-MS）测定的结果相关性良好。基于 Cu-BCS-AgNPs 的纸条的目视检测限为 0.06mg/L。我们的研究表明，这种方法有可能实现现场、快速且经济高效的食品和环境铜监测。
