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来自巴西东南部圣保罗州雅博蒂卡巴尔不同环境的外栖草蛉(脉翅目:草蛉科)线粒体细胞色素氧化酶亚基I基因的单倍型特征分析

Haplotype characterization of the COI mitochondrial gene in Chrysoperla externa (Neuroptera: Chrysopidae) from different environments in Jaboticabal, state of São Paulo, southeastern Brazil.

作者信息

Morales A C, Freitas S

机构信息

Departamento de Fitossanidade, Universidade Estadual Paulista, Jaboticabal, SP, Brazil.

出版信息

Braz J Biol. 2010 Nov;70(4):1115-21. doi: 10.1590/s1519-69842010000500030.

DOI:10.1590/s1519-69842010000500030
PMID:21180923
Abstract

The green lacewings (Chrysopidae) belong to the Order Neuroptera and are described as voracious predators in the larval stage and sometimes also in their adulthood. They are an important group used in integrated biological control in field and horticultural crops. Individuals of Chrysoperla externa were collected during 2007 until March 2008 in five different locations in Jaboticabal, SP, with all the seasons sampled. Thirty six sequences with 805 pairs of bases for the gene mitochondrial Citochrome Oxidase I (COI) were analysed. The genetic parameters revealed 24 haplotypes for this population, a total of 36 mutations and haplotype diversity of 0.956. The data of genetic distance and population structure calculated for this population considering the different areas and seasons, revealed a great genetic similarity and high degree of genetic sharing between individuals sampled. It showed that the species Chrysoperla externa from Jaboticabal, SP, is a single population, without genetic structure neither due to the area of origin nor to the seasons of the year.

摘要

草蛉(草蛉科)属于脉翅目,在幼虫阶段被描述为贪婪的捕食者,有时在成虫阶段也是如此。它们是用于田间和园艺作物综合生物防治的重要类群。2007年至2008年3月期间,在巴西圣保罗州雅博蒂卡巴尔的五个不同地点采集了外来草蛉个体,涵盖了所有季节。对线粒体细胞色素氧化酶I(COI)基因的36个序列(共805对碱基)进行了分析。遗传参数显示该种群有24个单倍型,共有36个突变,单倍型多样性为0.956。考虑到不同区域和季节,对该种群计算的遗传距离和种群结构数据显示,所采集个体之间具有很高的遗传相似性和遗传共享程度。结果表明,来自巴西圣保罗州雅博蒂卡巴尔的外来草蛉物种是一个单一的种群,无论起源区域还是一年中的季节,都不存在遗传结构。

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