Centro Nacional de Referencia de Control Biológico, Km 1.5, Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
Mol Biol Rep. 2019 Dec;46(6):6577-6583. doi: 10.1007/s11033-019-05034-9. Epub 2019 Aug 16.
The larvae of the Chrysoperla carnea-group (Neuroptera: Chrysopidae) are recognized among the most effective larval predators of various phytophagous arthropods. Therefore, green lacewings are commonly grown by commercial insectaries and released as biological control agents. Previous work has shown that commercial laboratories frequently supply indeterminate species of the large C. carnea cryptic species complex. In Mexico, at least 20 biological control companies have commercialized the species C. carnea, but none of the products reared by those companies have been analyzed scientifically. Thus, the goal of this work was to molecularly characterize nine C. carnea populations from Mexican insectaries using the most efficient molecular markers available: the mitochondrial genes COI, COII, ND2, and ND5. Phylogenetic analysis demonstrated a unique mitochondrial haplotype in seven commercial insectaries showing 100% similarity to the reference specimen C. plorabunda E100. In contrast, we observed two and four different mitochondrial haplotypes of the carnea-group in two commercial insectaries. More precisely, three specimens possessed the mitochondrial haplotype of the species C. zastrowi, suggesting possible natural occurrence of this haplotype in Mexico. Consequently, this study demonstrated the need for an extensive survey of the different laboratories and insectaries producing C. carnea in Mexico, including unambiguous species identification by song recordings to confirm the species identity of the observed mitochondrial haplotypes.
智利长足大蚕蛾组（Neuroptera：草蛉科）的幼虫被认为是各种植食性节肢动物最有效的幼虫捕食者之一。因此，绿僵菌通常由商业昆虫养殖场饲养，并作为生物防治剂释放。以前的工作表明，商业实验室经常提供不确定的大型智利长足大蚕蛾隐种复合体物种。在墨西哥，至少有 20 家生物防治公司已经将智利长足大蚕蛾商业化，但这些公司饲养的产品都没有经过科学分析。因此，这项工作的目的是使用最有效的分子标记物——线粒体基因 COI、COII、ND2 和 ND5——对来自墨西哥昆虫养殖场的 9 个智利长足大蚕蛾种群进行分子特征描述。系统发育分析表明，在七个商业昆虫养殖场中，有七个独特的线粒体单倍型，与参考标本 C. plorabunda E100 完全相似。相比之下，在两个商业昆虫养殖场中观察到了两个和四个不同的智利长足大蚕蛾组线粒体单倍型。更确切地说，三个标本具有 C. zastrowi 物种的线粒体单倍型，这表明这种单倍型可能在墨西哥自然发生。因此，这项研究表明，需要对墨西哥生产智利长足大蚕蛾的不同实验室和昆虫养殖场进行广泛调查，包括通过歌声记录进行明确的物种鉴定，以确认观察到的线粒体单倍型的物种身份。
