Trindade Lilian Cristine Teixeira, Biondo-Simões Maria de Lourdes Pessole, Sampaio Cláudia Paraguaçu Pupo, Farias Rogério Estevam, Pierin Rodrigo Jardim, Netto Miguel Chomiski
Post-Graduation in Surgical Clinic Program, Pontifical Catholic University of Paraná (PUCPR), Curitiba, Paraná, Brazil.
Rev Col Bras Cir. 2010 Oct;37(5):358-63. doi: 10.1590/s0100-69912010000500009.
To assess the efficacy of a topical 4% metronidazole solution in wound healing by secondary intention in rats.
We made circular wounds two inches in diameter at the back of rats and studied healing at 3, 7, 14 and 21 days. The wound contraction and epithelialization were assessed by peripheral digital planimetry and myofibroblasts by immunohistochemistry with a-SMA.
There was no difference between groups regarding wound contraction. In wounds treated with metronidazole peripheral epithelialization was evident early on day 3 (p <0.001) and there were no differences in other periods. In the control group, the number of myofibroblasts was higher on day 7 (p = 0.003) and day 14 (p = 0.001) and in the experimental group it was suggestively higher on day 3 (p = 0.06).
Metronidazole 4% solution at a dose of 50 mg/kg applied topically to wounds healing by secondary intention facilitates early peripheral epithelialization, does not interfere with wound contraction and delays the appearance of myofibroblasts.
评估局部应用4%甲硝唑溶液对大鼠二期愈合伤口愈合的疗效。
在大鼠背部制作直径2英寸的圆形伤口，并在第3、7、14和21天研究愈合情况。通过外周数字平面测量法评估伤口收缩和上皮化，通过α-SMA免疫组织化学法评估肌成纤维细胞。
各组间伤口收缩情况无差异。在甲硝唑治疗的伤口中，第3天早期外周上皮化明显（p<0.001），其他时期无差异。在对照组中，第7天（p=0.003）和第14天（p=0.001）肌成纤维细胞数量较高，而在实验组中，第3天肌成纤维细胞数量有升高趋势（p=0.06）。
以50mg/kg的剂量局部应用于二期愈合伤口的4%甲硝唑溶液可促进早期外周上皮化，不干扰伤口收缩，并延迟肌成纤维细胞的出现。
