Zailan N, Abdul Rashid A H, Das S, Abdul Mokti N A, Hassan Basri J, Teoh S L, Wan Ngah W Z, Mohd Yusof Y A
Departments of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Clin Ter. 2010;161(6):515-21.
Chlorella vulgaris (CV) is a green microalgae enriched with nutrients, vitamins, minerals and chlorophyll. The aim of our study was to evaluate the potential wound healing effects of CV as a dressing while comparing it to sodium alginate dressing.
Fifteen Sprague-Dawley rats were divided into three groups consisting of control (normal-wound with no dressing), CV and sodium alginate dressings. Wound was created on the dorsal surface of rats using a 6-mm sterilized punch biopsy. The wounds were examined on days 3, 6, 9 and 12 for dryness, exudation, contraction, period of epithelialization, complete wound closure and scar formation. All rats were sacrificed on day 12 and the granulation tissue formed on the wound was excised for the histological examination using Hematoxylin and Eosin stain (H&E).
The wound's size treated with CV dressing was significantly reduced (p<0.05) compared to the control and sodium alginate treated wounds. H&E staining showed that CV dressing minimized the formation of scar tissue during the healing process. Although there was no statistically significant difference in the complete wound closure and period of epithelialization among the groups, a significant difference was observed regarding the dryness, exudation and scar formation in the wound.
CV extract can be used as an effective supplement for wound dressing.
普通小球藻（CV）是一种富含营养物质、维生素、矿物质和叶绿素的绿色微藻。我们研究的目的是评估CV作为敷料的潜在伤口愈合效果，并将其与海藻酸钠敷料进行比较。
将15只Sprague-Dawley大鼠分为三组，分别为对照组（正常伤口，不使用敷料）、CV组和海藻酸钠敷料组。使用6毫米无菌打孔活检器在大鼠背部制造伤口。在第3、6、9和12天检查伤口的干燥情况、渗出情况、收缩情况、上皮化时间、伤口完全闭合情况和瘢痕形成情况。所有大鼠在第12天处死，切除伤口上形成的肉芽组织，用苏木精和伊红染色（H&E）进行组织学检查。
与对照组和海藻酸钠处理的伤口相比，用CV敷料处理的伤口大小显著减小（p<0.05）。H&E染色显示，CV敷料在愈合过程中使瘢痕组织的形成最小化。虽然各组之间在伤口完全闭合和上皮化时间方面没有统计学上的显著差异，但在伤口的干燥情况、渗出情况和瘢痕形成方面观察到了显著差异。
CV提取物可作为伤口敷料的有效补充剂。
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