Bari Elia, Arciola Carla Renata, Vigani Barbara, Crivelli Barbara, Moro Paola, Marrubini Giorgio, Sorrenti Milena, Catenacci Laura, Bruni Giovanna, Chlapanidas Theodora, Lucarelli Enrico, Perteghella Sara, Torre Maria Luisa
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Research Unit on Implant Infections, Rizzoli Orthopaedic Institute of Bologna, Via di Barbiano 1/10, 40136 Bologna, Italy.
Materials (Basel). 2017 Aug 23;10(9):983. doi: 10.3390/ma10090983.
Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by , while active polyphenols, polysaccharides and proteins are synthetized by and microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts ( and , Chl and Art, respectively) or silk sericin (Ser) or their mixtures (Chl-Ser and Art-Ser). The spray drying was a suitable method to produce microspheres with similar dimensions, characterized by collapsed morphology with a rough surface. Art and Art-Ser showed higher antioxidant properties than other formulations. All microspheres resulted in cytocompatibility on fibroblasts until 1.25 mg/mL and promoted cell migration and the complete wound closure; this positive effect was further highlighted after treatment with Art and Art-Ser. To our surprize the combination of sericin to Art did not improve the microalgae extract efficacy, at least in our experimental conditions.
由于其生物学特性，一些天然化合物最近在伤口愈合应用中得到了广泛应用。其中一种化合物是丝胶蛋白，它由 产生，而活性多酚、多糖和蛋白质则由 和微藻合成。我们的假设是，丝胶蛋白作为微胶囊化过程的最佳生物活性聚合物载体，也可以提高微藻的再生效果。结合无溶剂萃取法和喷雾干燥技术，基于藻类提取物（分别为 和 、Chl和Art）、丝胶蛋白（Ser）或它们的混合物（Chl-Ser和Art-Ser）获得了五种配方。喷雾干燥是一种生产尺寸相似的微球的合适方法，其特征是具有粗糙表面的塌陷形态。Art和Art-Ser表现出比其他配方更高的抗氧化性能。所有微球在浓度达到1.25 mg/mL时对成纤维细胞均具有细胞相容性，并促进细胞迁移和伤口完全闭合；在用Art和Art-Ser处理后，这种积极作用更加明显。令我们惊讶的是，至少在我们的实验条件下，丝胶蛋白与Art的组合并没有提高微藻提取物的功效。
